CS-0044

Mouse Naïve CD8+ T Cell Sorting Kit

Column-Based Negative Mouse

The Cell Sorts CS-0044 Mouse Naïve CD8 T Cell Sorting Kit uses column-assisted negative selection to isolate untouched naïve CD8+ T cells from mouse spleen with 90–99% purity and high recovery. Mouse Naïve CD8 T Cell Isolation Cocktail (10 μL) labels non-target cells at RT for 7.5–10 min. After wash/centrifugation (500×g, 5 min), Ultra-Nanobeads (10 μL) bind for 5 min. An L separation column depletes non-targets by gravity drip-through.

No bead or antibody contact with target cells. Untouched isolation preserves CD45RA+ CCR7+ naïve phenotype and full cytotoxic differentiation potential. Note: inflammatory models have lower naïve proportions — provide starting cell quantity. Ready for naïve CTL differentiation, primary cytotoxic response, tumor immunity, viral immunity, flow cytometry, scRNA-seq, and adoptive cell therapy.

Sorting Purity

90–99%

Sorting Time

25–35 min

Get a Quote Back to Products

Product Features of the Mouse Naïve CD8+ T Cell Sorting Kit

The Mouse Naïve CD8+ T Cell Sorting Kit delivers industry-leading performance for rapid Naïve CD8+ T Cell isolation from PBMCs via column-based negative selection.

Column-assisted separation — Magnetic column enables efficient depletion of non-target cells with high recovery rate

No bead residue on target cells — Isolated naïve CD8+ T cells are bead-free and ready for immediate downstream use

Purity 90–99% — High-efficiency negative selection for enriched naïve CD8+ T cell populations

High recovery rate — Optimized column format maximizes yield of viable naïve CD8+ T cells

Preserves naïve phenotype — Untouched isolation maintains CD45RA+ CCR7+ expression and full cytotoxic differentiation potential

Suitable for large samples — Column-based format handles larger cell numbers with consistent performance

Special note for inflammatory models — Naïve CD8 T cell proportions are lower in inflammatory mouse models; please provide starting cell quantity for optimal results

The Mouse Naïve CD8+ T Cell Sorting Kit uses column-based negative selection to isolate untouched Naïve CD8+ T Cells from human PBMCs. To achieve these results, follow this simple 4-step protocol that takes just 25–35 min from start to finish. No centrifugation and no lengthy incubations. Furthermore, this streamlined protocol ensures consistent results across experiments.

How the Mouse Naïve CD8+ T Cell Sorting Kit Works

Simple 4-step protocol for rapid cell isolation

Step 1: Sample Preparation

Grind mouse spleen through a 70 μm sterile nylon mesh filter to prepare a single-cell suspension. Count cells and resuspend 1×10⁷ cells in 100 μL FACS Buffer (scale proportionally for higher cell numbers).

Step 2: Antibody Labeling

Add 10 μL Mouse Naïve CD8 T Cell Isolation Cocktail to the sample, mix gently, and incubate at room temperature for 7.5–10 min to label non-naïve CD8⁺ T cells (CD4⁺ T cells, B cells, NK cells, monocytes, granulocytes, erythrocytes, memory/effector T cells, Tregs). Naïve CD8⁺ T cells remain completely untouched.

Step 3: Magnetic Bead Binding & Wash

Wash cells with 10× volume of FACS Buffer, centrifuge at 500×g for 5 min, and resuspend in 100 μL. Vortex Ultra-Nanobeads for 5–30 s, add 10 μL to the sample, mix gently, and incubate at room temperature for 5 min to bind labeled non-target cells.

Step 4: Column-Based Separation & Collection

Place an L separation column into the magnetic field and equilibrate with 3 mL FACS Buffer. Load the cell suspension onto the column and collect naïve CD8⁺ T cells as they drip through by gravity. Add another 3 mL FACS Buffer to wash the column and collect residual target cells for maximum yield.

Applications for the Mouse Naïve CD8+ T Cell Sorting Kit

This Mouse Naïve CD8+ T Cell Sorting Kit supports a wide range of downstream applications for Naïve CD8+ T Cell isolation and column-based negative selection workflows from mouse splenocytes or lymph node cells.

Naïve CD8+ T Cell Biology — De novo T cell activation, proliferation, and cytotoxic differentiation studies Cell Therapy R&D — CTL and CAR-T engineering from naïve CD8+ precursors for enhanced anti-tumor potency Vaccine Development — De novo CD8+ T cell immune response modeling and epitope discovery Infectious Disease Research — Pathogen-specific naïve CD8+ T cell priming and effector response analysis Flow Cytometry — Enriched naïve CD8+ T cell populations ready for CD8/CD45RA/CCR7 staining and analysis Cell Culture — Bead-free, unactivated naïve CD8+ T cells ideal for in vitro expansion and manipulation Cancer Immunology — Tumor antigen-specific naïve CD8+ T cell repertoire and differentiation studies

In addition, the Mouse Naïve CD8+ T Cell Sorting Kit is compatible with a wide range of downstream applications, making it a versatile tool for your immunology research workflow.

Kit Components

Each Mouse Naïve CD8+ T Cell Sorting Kit contains everything needed for high-purity column-based negative selection from human PBMCs.

Mouse naive CD8 T Cell Isolation Cocktail
Ultra-Nanobeads
User manual

Storage & Shelf Life

Storage

Store at 2-8°C protected from light

Shelf Life

12 months

Mouse Naïve CD8+ T Cell Sorting Kit — Frequently Asked Questions

Everything you need to know about the CS-0044 Mouse Naïve CD8+ T Cell Sorting Kit

Click each question to expand the answer

The CS-0044 Mouse Naïve CD8 T Cell Sorting Kit from Cell Sorts uses column-assisted negative selection. Mouse Naïve CD8 T Cell Isolation Cocktail (10 μL) labels non-naïve CD8⁺ T cells at room temperature for 7.5–10 min. After washing and centrifugation (500×g, 5 min), Ultra-Nanobeads (10 μL) bind labeled cells for 5 min. An L separation column in a magnetic field depletes non-targets by gravity drip-through. Naïve CD8⁺ T cells pass through untouched — bead-free and ready for downstream use.

Purity of isolated naïve CD8⁺ T cells typically reaches 90–99%, as validated by flow cytometry using CD8a-APC (Clone 53-6.7), CD62L-PE, CD44-FITC, CD45RA staining. The column-assisted format with wash/centrifugation step efficiently depletes memory/effector CD8⁺ T cells, B cells, CD4⁺ T cells, NK cells, monocytes, and granulocytes while maximizing recovery of viable naïve CD8⁺ T cells.

The CS-0044 kit is optimized for mouse spleen. Prepare a single-cell suspension by grinding through a 70 μm sterile nylon mesh filter. No red blood cell lysis is required. Resuspend 1×10⁷ cells in 100 μL FACS Buffer (scale proportionally for higher cell numbers). The kit is compatible with C57BL/6, BALB/c and other common mouse strains. Note: Naïve CD8 T cell proportions are lower in inflammatory mouse models; please provide starting cell quantity for optimal results.

Step 1: Sample Preparation. Grind mouse spleen through a 70 μm sterile nylon mesh filter. Count cells and resuspend 1×10⁷ cells in 100 μL FACS Buffer.
Step 2: Antibody Labeling. Add 10 μL Mouse Naïve CD8 T Cell Isolation Cocktail, mix gently, incubate at room temperature for 7.5–10 min.
Step 3: Magnetic Bead Binding & Wash. Wash cells with 10× volume of FACS Buffer, centrifuge at 500×g for 5 min, resuspend in 100 μL. Vortex Ultra-Nanobeads 5–30 s, add 10 μL, mix gently, incubate RT 5 min.
Step 4: Column-Based Separation & Collection. Place L separation column in magnetic field, equilibrate with 3 mL FACS Buffer. Load cell suspension, collect naïve CD8⁺ T cells as they drip through by gravity. Wash with 3 mL FACS Buffer for maximum yield.
Total hands-on time: ~25–30 min; total protocol: ~40–50 min.

Isolated naïve CD8⁺ T cells are immediately ready for: naïve CTL differentiation, primary cytotoxic response, tumor immunity, viral immunity studies, flow cytometry analysis, cell culture and expansion, CRISPR gene editing, single-cell RNA sequencing (scRNA-seq), and adoptive cell therapy studies. Bead-free and antibody-free output preserves full CD45RA⁺ CCR7⁺ expression and cytotoxic differentiation potential.

Antibodies and Ultra-Nanobeads bind exclusively to non-target cells — they never touch naïve CD8⁺ T cells. The untouched approach ensures full preservation of surface receptors (CD45RA, CCR7, CD62L), native functional states, and cytotoxic differentiation potential. The L separation column provides gentle, efficient depletion without mechanical stress on target cells.

The Mouse Naïve CD8 T Cell Isolation Cocktail (10 μL) targets CD4, CD19, CD11b, CD49b, Ly-6G, Ter-119, CD44, CD25 on non-target cells — effectively labeling CD4⁺ T cells, B cells, NK cells, monocytes, granulocytes, erythrocytes, memory/effector T cells, Tregs for depletion while leaving naïve CD8⁺ T cells (CD45RA⁺CCR7⁺CD62L⁺CD44⁻) completely untouched. This ensures 90–99% purity.

We recommend flow cytometry using CD8a-APC (Clone 53-6.7), CD62L-PE, CD44-FITC, CD45RA antibodies. Typical results show 90–99% naïve CD8⁺ T cells (CD62L⁺CD44⁻) in the collected fraction. Viability can be assessed using Trypan blue exclusion or 7-AAD staining. For best results, gate on lymphocytes by forward and side scatter, then analyze marker expression within the gated population.

Absolutely. Since isolated naïve CD8⁺ T cells are completely bead-free and antibody-free, they are immediately compatible with single-cell RNA sequencing (scRNA-seq) and other single-cell technologies without additional washing steps. Cell Sorts protocols are validated for 10x Genomics and other major scRNA-seq platforms.

The CS-0044 uses column-assisted negative selection (~25–30 min hands-on) with an L separation column and Ultra-Nanobeads, achieving 90–99% purity with high recovery for larger samples. It includes a centrifugation step (500×g, 5 min) for enhanced purity. The CS-0043 uses column-free negative selection (18–20 min) with Streptavidin Nanobeads, achieving >90% purity, up to 99% with maximum speed. Both preserve CD45RA⁺ CCR7⁺ naïve phenotype. Choose CS-0044 for larger-scale isolation; choose CS-0043 for rapid processing.