CellSorts CS-0037 Mouse Pan B Cell Sorting Kit — column-free negative selection, multi-tissue compatible, up to 98.8% purity
CS-0037

Mouse Pan B Cell Sorting Kit

Column-Free Negative Mouse

The Cell Sorts CS-0037 Mouse Pan B Cell Sorting Kit uses column-free negative selection to isolate untouched Pan B cells from mouse spleen, lymph nodes, bone marrow, and peritoneal lavage with up to 98.8% purity in ~25 min. Grind tissue through a 70 μm cell strainer and perform RBC lysis with ACK buffer. 2 μL Biotin-Antibody Mix labels non-B cells at 4°C for 10 min. After wash/centrifugation (500×g, 5 min), 20 μL Beads Streptavidin bind at 4°C for 10 min. Magnetic stand 5 min, then pour off purified Pan B cells. A final 500×g spin concentrates cells for downstream use.

No column, no bead or antibody contact with target cells. Multi-tissue compatible with high viability preservation through gentle handling. Isolated Pan B cells are free of surface antibody labeling — immediately ready for B cell activation, antibody production, BCR signaling, hybridoma generation, vaccine development, flow cytometry, scRNA-seq, and adoptive cell therapy.

Sorting Purity
Up to 98.8%
Sorting Time
~25 min

Product Features of the Mouse Pan B Cell Sorting Kit

The Mouse Pan B Cell Sorting Kit delivers industry-leading performance for rapid CD19+ Pan B Cell isolation from PBMCs via column-free negative selection.

Column-free separation — No separation column needed, streamlined magnetic separator workflow
Zero contact with target cells — Antibodies and beads bind non-B cells only, preserving Pan B cells in their native, unactivated state
Purity up to 98.8% — High-efficiency negative selection for ultra-pure Pan B cell populations from spleen
Multi-tissue compatible — Validated on mouse spleen, lymph nodes, bone marrow, and peritoneal lavage
No antibody labeling on B cells — Isolated cells are free of surface antibody, ready for immediate downstream use
~25 min fast sorting — Rapid protocol from sample preparation to purified Pan B cells
High viability — Gentle handling preserves cell integrity and functional activity

The Mouse Pan B Cell Sorting Kit uses column-free negative selection to isolate untouched CD19+ Pan B Cells from human PBMCs. To achieve these results, follow this simple 4-step protocol that takes just ~25 min from start to finish. No centrifugation and no lengthy incubations. Furthermore, this streamlined protocol ensures consistent results across experiments.

How the Mouse Pan B Cell Sorting Kit Works

Simple 4-step protocol for rapid cell isolation

1
Step 1 of pan B cells sorting: Sample Preparation - Grind mouse spleen, lymph node, or bone marrow through 70 μm strainer, ACK lysis, resuspend at 1×10⁸ cells/mL for CS-0037

Step 1: Sample Preparation

Grind mouse spleen, lymph node, or bone marrow through a 70 μm cell strainer, perform red blood cell lysis with ACK buffer, wash, filter again, count cells, and resuspend in separation buffer at 1×10⁸ cells/mL.

2
Step 2 of pan B cells sorting: Antibody Labeling - 2 μL Biotin-Antibody Mix, 4°C 10 min, wash 500×g, resuspend 100 μL separation buffer for CS-0037

Step 2: Antibody Labeling

Aliquot 100 μL cell suspension (1×10⁷ cells), add 2 μL Biotin-Antibody Mix, mix well, incubate at 4°C for 10 min. Wash with 1 mL separation buffer, centrifuge at 500×g for 5 min, resuspend in 100 μL separation buffer.

3
Step 3 of pan B cells sorting: Magnetic Bead Binding - 20 μL pre-washed Beads Streptavidin, 4°C 10 min to bind labeled non-B cells for CS-0037

Step 3: Magnetic Bead Binding

Add 20 μL pre-washed Beads Streptavidin, mix well, incubate at 4°C for 10 min to bind labeled non-B cells.

4
Step 4 of pan B cells sorting: Column-Free Magnetic Separation & Collection - 2.5 mL separation buffer, pipetting 5 times, magnetic stand 5 min, 500×g for CS-0037

Step 4: Column-Free Magnetic Separation & Collection

Dilute with 2.5 mL separation buffer, mix thoroughly by pipetting 5 times, place on magnetic stand for 5 min. Gently pour supernatant into sterile centrifuge tube while keeping tube on magnet. Centrifuge at 500×g for 5 min and resuspend for downstream use.

Applications for the Mouse Pan B Cell Sorting Kit

This Mouse Pan B Cell Sorting Kit supports a wide range of downstream applications for CD19+ Pan B Cell isolation and column-free negative selection workflows from mouse splenocytes or lymph node cells.

B Cell Biology Research — B cell activation, differentiation, proliferation, and antibody production studies Humoral Immunity Studies — Antigen-specific B cell response and immunoglobulin repertoire analysis Autoimmune Disease Models — B cell-mediated autoimmunity mechanism research (e.g., SLE, lupus) Infectious Disease Research — Pathogen-specific B cell response and neutralizing antibody development Flow Cytometry — High-purity Pan B cell populations ready for CD19 staining and analysis Cell Culture — Unactivated, antibody-free B cells ideal for in vitro expansion and manipulation Molecular Biology — Purified B cells for RNA/DNA extraction and gene expression analysis

In addition, the Mouse Pan B Cell Sorting Kit is compatible with a wide range of downstream applications, making it a versatile tool for your immunology research workflow.

Kit Components

Each Mouse Pan B Cell Sorting Kit contains everything needed for high-purity column-free negative selection from human PBMCs.

  • Magnetic bead-antibody cocktail
  • Sorting buffer
  • User manual

Storage & Shelf Life

Storage
Store at 2-8°C protected from light
Shelf Life
12 months

Mouse Pan B Cell Sorting Kit — Frequently Asked Questions

Everything you need to know about the CS-0037 Mouse Pan B Cell Sorting Kit

Click each question to expand the answer

The CS-0037 Mouse Pan B Cell Sorting Kit from Cell Sorts uses column-free negative selection. Biotin-Antibody Mix (2 μL) labels non-B cells (T cells, NK cells, monocytes, granulocytes, erythrocytes) at 4°C for 10 min. After wash/centrifugation (500×g, 5 min), Beads Streptavidin (20 μL) bind labeled cells at 4°C for 10 min. A magnetic stand (5 min) depletes non-targets; purified Pan B cells are poured off into a sterile centrifuge tube. A final 500×g centrifugation for 5 min concentrates cells for downstream use. No beads or antibodies ever contact the target cells.
Purity of isolated pan B cells typically reaches up to 98.8%, as validated by flow cytometry using CD19-PE (Clone 6D5), B220-APC, CD3-FITC staining. The optimized negative selection protocol with Biotin-Antibody Mix and Beads Streptavidin efficiently depletes all non-target cell populations while maximizing recovery of viable, functional Pan B cells from mouse spleen.
The CS-0037 kit is multi-tissue compatible — validated on mouse spleen, lymph nodes, bone marrow, and peritoneal lavage. Grind tissue through a 70 μm cell strainer, perform red blood cell lysis with ACK buffer, wash, filter again, count cells, and resuspend in separation buffer at 1×10⁸ cells/mL. The kit is compatible with C57BL/6, BALB/c and other common mouse strains used in immunology research.
Step 1: Sample Preparation. Grind mouse spleen, lymph node, or bone marrow through a 70 μm cell strainer, perform RBC lysis with ACK buffer, wash, filter again, count cells, and resuspend in separation buffer at 1×10⁸ cells/mL.
Step 2: Antibody Labeling. Aliquot 100 μL cell suspension (1×10⁷ cells), add 2 μL Biotin-Antibody Mix, mix well, incubate at 4°C for 10 min. Wash with 1 mL separation buffer, centrifuge at 500×g for 5 min, resuspend in 100 μL separation buffer.
Step 3: Magnetic Bead Binding. Add 20 μL pre-washed Beads Streptavidin, mix well, incubate at 4°C for 10 min.
Step 4: Column-Free Magnetic Separation & Collection. Dilute with 2.5 mL separation buffer, mix thoroughly by pipetting 5 times, place on magnetic stand for 5 min. Gently pour supernatant into sterile centrifuge tube while keeping tube on magnet. Centrifuge at 500×g for 5 min and resuspend for downstream use.
Total time: ~25 min.
Isolated pan B cells are immediately ready for: B cell activation, antibody production, BCR signaling, hybridoma generation, vaccine development, flow cytometry analysis, cell culture and expansion, CRISPR gene editing, single-cell RNA sequencing (scRNA-seq), and adoptive cell therapy studies. Zero bead or antibody interference means no additional washing steps are required.
Antibodies and Beads Streptavidin bind exclusively to non-target cells — they never touch Pan B cells. This untouched approach ensures full preservation of surface receptors (B220, CD19), native functional states, and physiological relevance for downstream functional assays. Cell Sorts column-free technology eliminates column-induced mechanical stress, further enhancing cell viability. High viability is maintained through gentle handling throughout the ~25 min protocol.
The Biotin-Antibody Mix (2 μL) targets CD3, CD4, CD8a, CD11b, CD49b, Ly-6G, Ter-119 on non-target cells — effectively labeling T cells, NK cells, monocytes, granulocytes, erythrocytes for depletion while leaving Pan B cells (CD19+ B220+) completely untouched. This comprehensive marker panel ensures up to 98.8% purity.
We recommend flow cytometry using CD19-PE (Clone 6D5), B220-APC, CD3-FITC antibodies. Typical results show up to 98.8% Pan B cells (CD19+ B220+) in the collected fraction. Viability can be assessed using Trypan blue exclusion or 7-AAD staining. For best results, gate on lymphocytes by forward and side scatter, then analyze marker expression within the gated population.
Absolutely. Since isolated Pan B cells are completely bead-free and antibody-free — with no antibody labeling on B cells — they are immediately compatible with single-cell RNA sequencing (scRNA-seq) and other single-cell technologies without additional washing steps. Cell Sorts protocols are validated for 10x Genomics and other major scRNA-seq platforms.
The CS-0037 uses column-free negative selection (~25 min) with 2 μL Biotin-Antibody Mix and 20 μL Beads Streptavidin, achieving up to 98.8% purity with multi-tissue compatibility (spleen, lymph nodes, bone marrow, peritoneal lavage). It includes wash/centrifugation steps (500×g, 5 min) for enhanced purity. Column-based kits (25–35 min) offer higher consistency for larger sample sizes. Both yield untouched, bead-free Pan B cells. Choose CS-0037 for multi-tissue flexibility and up to 98.8% purity; choose column-based for larger-scale isolation.

Related topics covered:

column-free negative selection CD19+ Pan B Cell isolation CD19+ Pan B Cell purity verification CD19+ Pan B Cell sorting protocol column-free isolation single-cell analysis (scRNA-seq) CD19+ Pan B Cell isolation markers PBMC CD19+ Pan B Cell sorting

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