CS-0049

Mouse CD19 NanoBeads Sorting Kit

Column-Based Positive Mouse

The Cell Sorts Mouse CD19 NanoBeads Sorting Kit (CS-0049) uses column-based positive selection to isolate B lymphocytes expressing the CD19 surface marker from mouse splenocytes or lymph node cell suspensions with 95–99% purity in 20–30 min. Anti-mouse NanoBeads conjugated to the pan-B cell marker bind CD19⁺ populations, which are retained in a magnetic separation column; unlabeled non-target cells (T lymphocytes, NK cells, monocytes, granulocytes) flow through. Eluted B lineage cells show >90% recovery and >98% viability — ready for B cell activation assays, hybridoma generation, antibody production, and BCR repertoire sequencing.

Compared to density gradient centrifugation (40–60% purity) or FACS (expensive, low throughput), this Cell Sorts kit delivers 95–99% purity with >90% recovery. The releasable NanoBeads leave cells bead-free in 10 min — ideal for single-cell RNA sequencing (scRNA-seq), electroporation, and high-throughput monoclonal antibody screening. Optimized for C57BL/6, BALB/c, and common mouse strains.

Sorting Purity

≥95%

Sorting Time

~30 min

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Product Features of the Mouse CD19 NanoBeads Sorting Kit

The Mouse CD19 NanoBeads Sorting Kit delivers industry-leading performance for rapid CD19+ B Cell isolation from PBMCs via column-based positive selection.

Column-assisted positive selection — Anti-mouse CD19 antibody-conjugated NanoBeads directly label CD19+ B cells for efficient magnetic capture in a separation column from mouse splenocytes

Purity 95–99% — Single-pass positive selection delivers ultra-pure CD19+ B cell populations from mouse single-cell suspensions, validated by flow cytometry

Releasable from column — Elute captured CD19+ B cells by plunger press; optional Release Buffer removes NanoBeads for bead-free downstream processing

B cell-specific targeting — CD19 is expressed from pro-B cell through mature B cell stages, making it an ideal pan-B cell marker for comprehensive B cell isolation

Scalable format — Medium column accommodates up to 1×10⁹ total cells; from bench-scale immunology to large-scale antibody screening and hybridoma generation

Flow cytometry validated — Compatible with CD19-PE (Clone 6D5) and B220-APC staining for immediate purity verification and B cell subset characterization

The Mouse CD19 NanoBeads Sorting Kit uses column-based positive selection to isolate purified CD19+ B Cells from human PBMCs. To achieve these results, follow this simple 4-step protocol that takes just ~30 min from start to finish. No centrifugation and no lengthy incubations. Furthermore, this streamlined protocol ensures consistent results across experiments.

How the Mouse CD19 NanoBeads Sorting Kit Works

Simple 4-step protocol for rapid cell isolation

Step 1: Sample Preparation

Prepare single-cell suspension from mouse spleen/lymph nodes. Pass through 70 μm strainer, perform RBC lysis if needed. Count cells, resuspend 1×10⁷ cells in 90 μL cold separation buffer (2–8°C). Add FcR blocker to prevent non-specific binding.

Step 2: Magnetic Bead Labeling

Add 10 μL Mouse CD19 NanoBeads, mix thoroughly, incubate 4°C for 10 min. Anti-mouse CD19 NanoBeads specifically label CD19⁺ B cells. Wash with 1–2 mL separation buffer, centrifuge 300×g for 10 min, resuspend in 1 mL separation buffer.

Step 3: Column-Based Magnetic Separation

Place medium column in magnetic separator, equilibrate with 3 mL buffer. Load cell suspension, allow flow-through, wash once with 3 mL buffer to remove unlabeled non-target cells. Magnetically labeled CD19⁺ B cells are retained in the column.

Step 4: Target Cell Elution & Collection

Remove column from magnet, place over collection tube, add 3 mL separation buffer, flush CD19⁺ B cells with plunger. For bead-free cells, add Release Buffer (4°C, 10 min). Ready for flow cytometry, B cell activation, or hybridoma generation.

Applications for the Mouse CD19 NanoBeads Sorting Kit

This Mouse CD19 NanoBeads Sorting Kit supports a wide range of downstream applications for CD19+ B Cell isolation and column-based positive selection workflows from mouse splenocytes or lymph node cells.

B Cell Biology Research — B cell activation, differentiation, proliferation, and antibody production studies in mouse models Humoral Immunity Studies — Antigen-specific B cell response and immunoglobulin repertoire analysis B Cell Malignancy Research — B cell lymphoma and acute lymphoblastic leukemia model studies (CD19 is highly expressed on malignant B cells) CAR-T Development — CD19-targeted CAR-T therapy preclinical evaluation Flow Cytometry — High-purity CD19+ B cell populations ready for multi-parameter staining and analysis Cell Culture — Bead-bound CD19+ B cells for activation, expansion, and functional manipulation Drug Discovery — B cell-targeted drug screening in syngeneic and transgenic mouse models

In addition, the Mouse CD19 NanoBeads Sorting Kit is compatible with a wide range of downstream applications, making it a versatile tool for your immunology research workflow.

Kit Components

Each Mouse CD19 NanoBeads Sorting Kit contains everything needed for high-purity column-based positive selection from human PBMCs.

Mouse CD19 Nano Beads
User manual

Storage & Shelf Life

Storage

Store at 2-8°C protected from light

Shelf Life

12 months

Mouse CD19 NanoBeads Sorting Kit — Frequently Asked Questions

Everything you need to know about the CS-0049 Mouse CD19 NanoBeads Sorting Kit

Click each question to expand the answer

The CS-0049 Mouse CD19 NanoBeads Sorting Kit from Cell Sorts uses column-based positive selection. Anti-mouse CD19 antibody-conjugated NanoBeads directly bind to the CD19 surface marker expressed on B lineage cells. The cell-bead mixture is applied to a magnetic separation column, where CD19⁺ B cells are retained. Unlabeled non-target cells flow through. The column is then removed from the magnet, and purified B cells are eluted by plunger press.

Purity of isolated mouse B cells typically reaches 95–99%, as validated by flow cytometry using anti-mouse CD19 antibody staining. The NanoBeads positive selection protocol efficiently enriches B lymphocytes from mouse splenocytes and lymph node cells while depleting T cells, NK cells, and myeloid cells.

The CS-0049 kit is optimized for mouse splenocytes and lymph node cell suspensions. Prepare a single-cell suspension by gentle mechanical disruption through a 70 μm cell strainer. Perform red blood cell lysis if needed (e.g., ACK lysis buffer). The kit is compatible with C57BL/6, BALB/c, and other common mouse strains used in immunology research.

Step 1: Prepare a single-cell suspension from mouse spleen or lymph nodes.
Step 2: Count cells and resuspend at recommended density.
Step 3: Incubate with anti-mouse CD19 NanoBeads at 4°C for 10 min.
Step 4: Load onto pre-washed magnetic column.
Step 5: CD19⁺ B cells are retained; unlabeled cells flow through.
Step 6: Elute purified B cells by plunger press.
Total time: 20–30 minutes.

Isolated mouse B cells are immediately ready for: B cell activation and proliferation assays, antibody production and secretion studies, BCR signaling analysis, cytokine profiling (IL-6, IL-10), hybridoma generation for monoclonal antibody production, single-cell RNA sequencing (scRNA-seq), BCR repertoire sequencing, vaccine development studies, infectious disease research, autoimmune disease models (SLE, RA), and CRISPR gene editing.

Yes, the CS-0049 kit is ideal for hybridoma generation. The high purity (95–99%) and bead-free nature of isolated B cells provide optimal starting material for cell fusion with myeloma cells. The gentle positive selection protocol preserves B cell viability and functionality, ensuring high fusion efficiency and antibody-producing hybridoma yield. Cell Sorts recommends this kit for monoclonal antibody development workflows.

The CS-0049 kit uses nano-sized magnetic beads (NanoBeads) that offer higher binding efficiency and lower non-specific binding compared to conventional micron-sized beads. Their small size ensures minimal interference with the B cell receptor (BCR) complex and signaling pathways, and they are fully releasable — leaving B cells bead-free for sensitive downstream functional assays.

We recommend flow cytometry using anti-mouse CD19-PE (Clone 6D5), B220-APC, CD3-FITC, and CD11b-PerCP-Cy5.5 antibodies. Typical results show >95% CD19⁺B220⁺ cells with minimal CD3⁺ T cell or CD11b⁺ myeloid contamination. Additional markers (IgD, CD27, CD38, GL7) can be used to distinguish follicular, marginal zone, germinal center, and plasma B cell subsets.

Absolutely. Since isolated mouse B cells are bead-free after NanoBeads release, they are immediately compatible with single-cell RNA sequencing (scRNA-seq), BCR repertoire sequencing, and other single-cell technologies. This enables high-resolution profiling of B cell heterogeneity and antibody repertoire analysis at single-cell resolution in mouse models. Cell Sorts protocols are validated for 10x Genomics and other major scRNA-seq platforms.

The CS-0049 kit targets mouse CD19⁺ B cells using anti-mouse CD19 antibodies, designed for mouse splenocytes and lymph node cells in murine immunology research. The CS-0025 kit targets human CD19⁺ B cells using anti-human CD19 antibodies, designed for human PBMCs in clinical and translational research. Both use column-based positive selection with releasable NanoBeads and achieve 95–99% purity.