MBCS-0013

Anti-FITC Sorting Magnetic Beads

Other Human peripheral blood, other tissues

Anti-FITC Sorting Magnetic Beads provide a flexible, antibody-directed solution for magnetic cell isolation when targets are labeled with a FITC-conjugated antibody. This format helps researchers build a reproducible workflow around the FITC antibody already used in their assay. The beads support both positive selection, in which FITC-labeled target cells are retained and collected, and negative selection, in which unwanted FITC-labeled cells are depleted while the desired cells are recovered in the flow-through. The workflow is designed for cold labeling at 2–8 °C and uses a standard magnetic column for efficient separation. After FITC antibody staining, cells are incubated with Anti-FITC magnetic beads, loaded onto a pre-rinsed column, washed, and—when performing positive selection—eluted for collection. The resulting cell populations can proceed to flow cytometry, cell culture, functional assays, and genomic or transcriptomic studies.

Our Anti-FITC Sorting Magnetic Beads is designed for researchers who need reliable column-based positive selection from human PBMCs with high purity and minimal processing time. As a result, researchers can obtain highly purified Target Cell without separation hassles.

Product Specifications

Particle Size

50 nm

Sorting Method

Column-Based

Sorting Principle

Positive&Negative

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Core Advantages of the Anti-FITC Sorting Magnetic Beads

The Anti-FITC Sorting Magnetic Beads delivers industry-leading performance for rapid Target Cell enrichment from Human peripheral blood, other tissues samples via column-based positive selection.

High purity 95-99% — Advanced negative selection for ultra-pure cell populations

Fast protocol — Complete isolation in just 25-35 min

Column-assisted separation — Magnetic column enables efficient depletion with high recovery rate

No bead residue — Isolated cells are bead-free and ready for immediate downstream use

Preserved functionality — Antibodies and beads do not contact target cells, maintaining their original functional state

The Anti-FITC Sorting Magnetic Beads uses column-based positive selection to enrich purified Target Cells from Human peripheral blood, other tissues samples. To achieve these results, follow this simple 4-step protocol that takes just 25-35 min from start to finish. Furthermore, this streamlined protocol ensures consistent results across experiments.

How the Anti-FITC Sorting Magnetic Beads Works

Simple 4-step protocol for rapid cell isolation

1

Step 1: Sample Preparation

Prepare a single-cell suspension from your tissue sample, filter to remove clumps, and resuspend at the recommended cell concentration.

2

Step 2: Antibody Labeling

Incubate cells with target-specific antibodies at 4°C for 5–10 min to label target cells for positive selection.

3

Step 3: Magnetic Capture

Add magnetic beads and incubate for 5–10 min; beads bind to labeled target cells, magnetically tagging them for capture.

4

Step 4: Magnetic Separation & Collection

Place in magnetic separator; bead-bound target cells are retained while non-target cells are washed away. Purified target cells are then eluted and ready for downstream applications.

Applications for the Anti-FITC Sorting Magnetic Beads

This Anti-FITC Sorting Magnetic Beads supports a wide range of downstream applications for Target Cell isolation and column-based positive selection workflows from human PBMCs.

FITC antibody-guided enrichment of target cell populations Depletion of unwanted FITC-labeled cells Flow cytometry sample preparation Cell culture and functional assays Genomic and transcriptomic downstream studies

In addition, the Anti-FITC Sorting Magnetic Beads is compatible with a wide range of downstream applications, making it a versatile tool for your immunology research workflow.

Applicable Samples

The Anti-FITC Sorting Magnetic Beads is validated for high-purity column-based positive selection from the following Human peripheral blood, other tissues sample types.

Human peripheral blood
other tissues

Storage & Shelf Life

Storage

2-8°C

Shelf Life

12 months

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Anti-FITC Sorting Magnetic Beads — Frequently Asked Questions

Everything you need to know about the MBCS-0013 Anti-FITC Sorting Magnetic Beads

Click each question to expand the answer

This kit uses column-based negative selection. Non-target cells are labeled and retained in the magnetic column, while untouched target cells pass through by gravity into the collection tube. This strategy ensures no beads or antibodies ever contact the target cells.

Purity exceeds 90% and reaches up to 99%, as validated by flow cytometry analysis.

Antibodies and streptavidin nanobeads bind exclusively to non-target cells—they never touch the target cells. This ensures full preservation of surface receptors and native functional states.

1) Add FcR Blocker to sample. 2) Incubate with antibody cocktail. 3) Add streptavidin nanobeads. 4) Pass through magnetic column. 5) Collect untouched target cells. Total time: 25-35 minutes.

Isolated cells are ready for flow cytometry, cell culture, functional assays, gene editing, single-cell sequencing, and drug screening.