MBCS-0012

Anti-Biotin Sorting Magnetic Beads

Other Human peripheral blood, other tissues

Anti-Biotin Sorting Magnetic Beads are generated by covalently conjugating Anti-Biotin monoclonal antibody onto biodegradable nanoscale magnetic beads (50–100 nm). The beads specifically bind to biotinylated molecules without binding free biotin. Users can pair any biotinylated antibody of choice to achieve gentle sorting, obtaining target cells with high purity, high viability, and high recovery — seamlessly compatible with downstream culture, sequencing, and other assays.

Our Anti-Biotin Sorting Magnetic Beads is designed for researchers who need reliable column-free positive selection from human PBMCs with high purity and minimal processing time. As a result, researchers can obtain highly purified Target Cell without separation hassles.

Product Specifications

Particle Size

50–100 nm

Sorting Method

Column-Free

Sorting Principle

Positive

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Core Advantages of the Anti-Biotin Sorting Magnetic Beads

The Anti-Biotin Sorting Magnetic Beads delivers industry-leading performance for rapid Target Cell enrichment from Human peripheral blood, other tissues samples via column-free positive selection.

High purity 95-99% — Advanced negative selection for ultra-pure cell populations

Fast protocol — Complete isolation in just 25-35 min

Column-free — No columns or centrifugation required, simplifying your workflow

No bead residue — Isolated cells are bead-free and ready for immediate downstream use

Preserved functionality — Antibodies and beads do not contact target cells, maintaining their original functional state

The Anti-Biotin Sorting Magnetic Beads uses column-free positive selection to enrich purified Target Cells from Human peripheral blood, other tissues samples. To achieve these results, follow this simple 4-step protocol that takes just 18-20 min from start to finish. Furthermore, this streamlined protocol ensures consistent results across experiments.

How the Anti-Biotin Sorting Magnetic Beads Works

Simple 4-step protocol for rapid cell isolation

1

Step 1: Sample Preparation

Prepare a PBMC single-cell suspension, adjust cell concentration to 1×10⁸ cells/mL, and add FcR Blocker to prevent non-specific binding.

2

Step 2: Antibody Cocktail Incubation

Incubate cells with the biotin-conjugated antibody cocktail at 4°C for 5–10 min to label non-target cells, leaving target cells untouched.

3

Step 3: Magnetic Nanobead Binding

Add streptavidin nanobeads and incubate for 5–10 min; nanobeads bind to labeled non-target cells, magnetically tagging them for removal.

4

Step 4: Column-Free Separation & Collection

Place the tube in a magnetic separator for 2–3 min to pull non-target cells to the wall, then simply pour off the untouched, purified target cells into a fresh tube.

Applications for the Anti-Biotin Sorting Magnetic Beads

This Anti-Biotin Sorting Magnetic Beads supports a wide range of downstream applications for Target Cell isolation and column-free positive selection workflows from human PBMCs.

Positive enrichment of any target cell population using a user-selected biotinylated antibody (e.g., biotinylated anti-CD3, anti-CD4, anti-CD8, etc.) Flexible isolation of rare or custom cell subsets from PBMCs or tissue suspensions Cell isolation from peripheral blood, leukapheresis products, or single-cell tissue suspensions Downstream applications: cell culture, single-cell RNA sequencing (scRNA-seq), genomic/transcriptomic analysis, flow cytometry, functional assays, adoptive cell therapy research

In addition, the Anti-Biotin Sorting Magnetic Beads is compatible with a wide range of downstream applications, making it a versatile tool for your immunology research workflow.

Applicable Samples

The Anti-Biotin Sorting Magnetic Beads is validated for high-purity column-free positive selection from the following Human peripheral blood, other tissues sample types.

Human peripheral blood
other tissues

Storage & Shelf Life

Storage

2-8°C

Shelf Life

12 months

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Anti-Biotin Sorting Magnetic Beads — Frequently Asked Questions

Everything you need to know about the MBCS-0012 Anti-Biotin Sorting Magnetic Beads

Click each question to expand the answer

Our kits consistently achieve 95-98% purity, as validated by flow cytometry analysis.

The entire procedure takes approximately 18-20 minutes from start to finish, including incubation and separation steps.

Yes, our column-free negative selection technology uses no beads on the target cells. Only the unwanted cells carry the magnetic beads, which are discarded.

This kit is designed for the specified target cell isolation from PBMCs. Please refer to the product specifications for detailed target cell information.

Yes, the isolated cells maintain high viability and functional integrity, making them suitable for flow cytometry, cell culture, RNA sequencing, adoptive cell therapy, and other downstream applications.