MBCS-0010

Human CD8 Sorting Magnetic Beads

CD Beads Human peripheral blood, Leukapheresis products, Other tissues

The Cell Sorts MBCS-0010 Human CD8 Sorting Magnetic Beads uses column-based positive selection to enrich and purify CD8+ cytotoxic T cells from human PBMCs with high purity in ~30–40 min. The high-specificity anti-human CD8 monoclonal antibody-conjugated nanobeads (50–100 nm) specifically label CD8+ CTLs, which are retained on a pre-rinsed magnetic column while unlabeled non-target cells flow through. Isolated cells are ready for flow cytometry, CTL assays, cancer immunotherapy, and cell culture.

20 μL CD8 magnetic beads per 1×10⁷ cells, incubated at 2–8°C for 15 min, delivers high-purity CD8+ T cell populations. The streamlined MACS workflow with established protocols ensures consistent results across experiments. The biodegradable nanoscale matrix minimizes steric hindrance and preserves cell viability and cytotoxic functional integrity.

Product Specifications

Particle Size

50–100 nm

Sorting Method

Column-Based

Sorting Principle

Positive

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Core Advantages of the Human CD8 Sorting Magnetic Beads

The Human CD8 Sorting Magnetic Beads delivers industry-leading performance for rapid CD8+ Cytotoxic T Cell enrichment from Human peripheral blood, Leukapheresis products, Other tissues samples via column-based positive selection.

Biodegradable nanoscale matrix (50–100 nm) — minimizes steric hindrance and mechanical stress on cells

High-specificity anti-human CD8 monoclonal antibody — ensures efficient capture of CD8+ cytotoxic T cells

High-purity enrichment and isolation of CD8+ T cells from PBMCs

Gentle sorting — preserves cell viability and functional integrity

Streamlined MACS workflow — with established protocols for consistent results

No column clogging — due to nanoscale bead size

The Human CD8 Sorting Magnetic Beads uses column-based positive selection to enrich purified CD8+ Cytotoxic T Cells from Human peripheral blood, Leukapheresis products, Other tissues samples. To achieve these results, follow this simple 4-step protocol that takes just 25-35 min from start to finish. Furthermore, this streamlined protocol ensures consistent results across experiments.

How the Human CD8 Sorting Magnetic Beads Works

Simple 4-step protocol for rapid cell isolation

Step 1: Sample Preparation

Prepare human PBMCs, count the cells, transfer 1×10⁷ cells into a tube, and centrifuge at 1500 rpm for 5 min.

Step 2: Magnetic Labeling

Resuspend the cell pellet in 100 μL sorting buffer, add 20 μL CD8 magnetic beads, mix thoroughly, and incubate at 2–8°C for 15 min.

Step 3: Magnetic Separation

Load the sample onto a magnetic column pre-rinsed with 3 mL buffer, collect the flow-through, and wash the column three times with 3 mL sorting buffer to remove unlabeled cells.

Step 4: Elution

Remove the column from the magnet, add 5 mL sorting buffer, immediately push the plunger through, and collect the eluted CD8+ target cells.

Applications for the Human CD8 Sorting Magnetic Beads

This Human CD8 Sorting Magnetic Beads supports a wide range of downstream applications for CD8+ Cytotoxic T Cell isolation and column-based positive selection workflows from human PBMCs.

Positive enrichment and purification of CD8+ cytotoxic T lymphocytes (CTLs) from human peripheral blood PBMCs Isolation of CD8+ T cells from leukapheresis products CD8+ T cell enrichment for downstream functional studies (cytotoxicity, killing assays), expansion, and activation Depletion of CD8+ T cells from mixed cell populations Downstream applications: cell culture, flow cytometry analysis, CTL functional assays, genomic/transcriptomic studies, adoptive cell therapy research

In addition, the Human CD8 Sorting Magnetic Beads is compatible with a wide range of downstream applications, making it a versatile tool for your immunology research workflow.

Applicable Samples

The Human CD8 Sorting Magnetic Beads is validated for high-purity column-based positive selection from the following Human peripheral blood, Leukapheresis products, Other tissues sample types.

Human peripheral blood
Leukapheresis products
Other tissues

Storage & Shelf Life

Storage

2-8°C

Shelf Life

12 months

Human CD8 Sorting Magnetic Beads — Frequently Asked Questions

Everything you need to know about the MBCS-0010 Human CD8 Sorting Magnetic Beads

Click each question to expand the answer

The MBCS-0010 Human CD8 Sorting Magnetic Beads from Cell Sorts uses column-based positive selection. 20 μL CD8 magnetic beads are added to 1×10⁷ cells resuspended in 100 μL sorting buffer and incubated at 2–8°C for 15 min. The sample is loaded onto a magnetic column pre-rinsed with 3 mL buffer, the flow-through is collected, and the column is washed three times with 3 mL sorting buffer. The column is then removed from the magnet, 5 mL sorting buffer is added, and the plunger is pushed through to elute purified CD8+ target cells.

The MBCS-0010 kit delivers high-purity enrichment and isolation of CD8+ T cells from PBMCs, as validated by flow cytometry using CD8-PE (Clone SK1), CD3-APC, CD4-FITC staining. The high-specificity anti-human CD8 monoclonal antibody ensures efficient capture with minimal non-specific carryover. Purity typically reaches 90–99%.

The MBCS-0010 kit is optimized for human PBMCs. Prepare human PBMCs, count the cells, transfer 1×10⁷ cells into a tube, and centrifuge at 1500 rpm for 5 min. The kit is compatible with PBMCs from healthy donors and patients with various conditions.

Step 1: Sample Preparation. Prepare human PBMCs, count cells, transfer 1×10⁷ cells into a tube, and centrifuge at 1500 rpm for 5 min.
Step 2: Magnetic Labeling. Resuspend the cell pellet in 100 μL sorting buffer, add 20 μL CD8 magnetic beads, mix thoroughly, and incubate at 2–8°C for 15 min.
Step 3: Magnetic Separation. Load the sample onto a magnetic column pre-rinsed with 3 mL buffer, collect the flow-through, and wash the column three times with 3 mL sorting buffer.
Step 4: Elution. Remove the column from the magnet, add 5 mL sorting buffer, immediately push the plunger through, and collect the eluted CD8+ target cells.
Total time: ~30–40 min.

Isolated CD8+ T cells are immediately ready for: cancer immunotherapy (CTL assays, TIL studies, adoptive cell therapy), viral immunology, flow cytometry, cell culture and expansion, functional assays (cytotoxicity, degranulation), genomic analysis (RNA-seq, scRNA-seq), CAR-T development, and drug discovery. The gentle sorting protocol preserves cell viability and functional integrity.

The high-specificity anti-human CD8 monoclonal antibody ensures efficient capture of CD8+ cytotoxic T cells with minimal off-target binding. CD8 is a well-established marker for cytotoxic T lymphocytes, and this antibody clone provides consistent, reliable capture of target cells, resulting in high-purity enriched populations while preserving native surface markers and functional integrity.

The biodegradable nanoscale matrix (50–100 nm) minimizes steric hindrance and mechanical stress on cells compared to larger microbeads. This ensures efficient antibody-target binding while preserving native cell surface markers, viability, and cytotoxic functional integrity — critical for downstream CTL assays and functional studies. Additionally, the nanoscale bead size prevents column clogging, ensuring smooth and consistent separation performance.

We recommend flow cytometry using CD8-PE (Clone SK1), CD3-APC, CD4-FITC antibodies. Typical results show 90–99% CD8+ cells (CD8+ CD3+ CD4−) in the positively selected fraction. Viability can be assessed using Trypan blue exclusion or 7-AAD staining. For cytotoxic function verification, perforin/granzyme B staining or in vitro killing assays can be performed.

Absolutely. Isolated CD8+ T cells are immediately ready for single-cell RNA sequencing (scRNA-seq) without additional washing. The gentle 50–100 nm nanobeads and streamlined MACS workflow preserve transcriptomic integrity, making the MBCS-0010 kit compatible with 10x Genomics and other major scRNA-seq platforms.

The MBCS-0010 Human CD8 Sorting Magnetic Beads should be stored at 2–8°C and used within 12 months of opening. Do not freeze. The streamlined MACS workflow with established protocols ensures consistent results across experiments.