MBCS-0008

Human CD4 Sorting Magnetic Beads

CD Beads Human peripheral blood, Leukapheresis products, Other tissues

The Cell Sorts MBCS-0008 Human CD4 Sorting Magnetic Beads uses column-based positive selection to enrich and purify CD4+ T cells from human PBMCs. The high-specificity anti-human CD4 monoclonal antibody ensures efficient capture. Isolate PBMCs, filter through a 30 μm cell strainer, and centrifuge 1×10⁷ cells at 1500 rpm for 5 min. Resuspend in 100 μL sorting buffer, add 20 μL CD4 magnetic beads, and incubate at 2–8°C for 15 min. Load onto a pre-rinsed magnetic column, wash three times with 3 mL sorting buffer, then elute with 5 mL buffer via plunger.

The biodegradable nanoscale matrix (50–100 nm) minimizes steric hindrance and mechanical stress, preserving cell viability and functional integrity. No column clogging due to nanoscale bead size. Isolated CD4+ T cells are ready for immunology research, flow cytometry, cell culture, inflammation studies, scRNA-seq, and cancer immunotherapy.

Product Specifications

Particle Size

50–100 nm

Sorting Method

Column-Based

Sorting Principle

Positive&Negative

Get a Quote Back to Products

Core Advantages of the Human CD4 Sorting Magnetic Beads

The Human CD4 Sorting Magnetic Beads delivers industry-leading performance for rapid CD4+ T Cell enrichment from Human peripheral blood, Leukapheresis products, Other tissues samples via column-based positive selection.

Biodegradable nanoscale matrix (50–100 nm) — minimizes steric hindrance and mechanical stress on cells

High-specificity anti-human CD4 monoclonal antibody — ensures efficient capture of CD4+ T cells

High-purity enrichment and isolation of CD4+ T cells from PBMCs

Gentle sorting — preserves cell viability and functional integrity

Streamlined MACS workflow — with established protocols for consistent results

No column clogging — due to nanoscale bead size

The Human CD4 Sorting Magnetic Beads uses column-based positive selection to enrich purified CD4+ T Cells from Human peripheral blood, Leukapheresis products, Other tissues samples. To achieve these results, follow this simple 4-step protocol that takes just 25-35 min from start to finish. Furthermore, this streamlined protocol ensures consistent results across experiments.

How the Human CD4 Sorting Magnetic Beads Works

Simple 4-step protocol for rapid cell isolation

Step 1: Sample Preparation

Isolate PBMCs from human peripheral blood, filter through a 30 μm cell strainer, count cells, and centrifuge 1×10⁷ cells at 1500 rpm for 5 min.

Step 2: Magnetic Labeling

Resuspend the cell pellet in 100 μL sorting buffer, add 20 μL CD4 magnetic beads, mix thoroughly, and incubate at 2–8°C for 15 min.

Step 3: Magnetic Separation

Load the sample onto a pre-rinsed magnetic column, collect the flow-through, and wash the column three times with 3 mL sorting buffer to remove unlabeled cells.

Step 4: Elution

Remove the column from the magnet, add 5 mL sorting buffer, immediately push the plunger through, and collect the eluted CD4+ target cells.

Applications for the Human CD4 Sorting Magnetic Beads

This Human CD4 Sorting Magnetic Beads supports a wide range of downstream applications for CD4+ T Cell isolation and column-based positive selection workflows from human PBMCs.

Positive enrichment and purification of CD4+ T cells from human peripheral blood PBMCs Isolation of CD4+ T cells from leukapheresis products CD4+ T cell enrichment for downstream functional studies, expansion, and activation assays Depletion of CD4+ T cells from mixed cell populations Downstream applications: cell culture, flow cytometry analysis, T cell functional assays (proliferation, cytokine profiling), genomic/transcriptomic studies, adoptive cell therapy research

In addition, the Human CD4 Sorting Magnetic Beads is compatible with a wide range of downstream applications, making it a versatile tool for your immunology research workflow.

Applicable Samples

The Human CD4 Sorting Magnetic Beads is validated for high-purity column-based positive selection from the following Human peripheral blood, Leukapheresis products, Other tissues sample types.

Human peripheral blood
Leukapheresis products
Other tissues

Storage & Shelf Life

Storage

2-8°C

Shelf Life

12 months

Human CD4 Sorting Magnetic Beads — Frequently Asked Questions

Everything you need to know about the MBCS-0008 Human CD4 Sorting Magnetic Beads

Click each question to expand the answer

The MBCS-0008 Human CD4 Sorting Magnetic Beads from Cell Sorts uses column-based positive selection. 20 μL CD4 magnetic beads are added to 1×10⁷ cells resuspended in 100 μL sorting buffer and incubated at 2–8°C for 15 min. The sample is loaded onto a pre-rinsed magnetic column, the flow-through is collected, and the column is washed three times with 3 mL sorting buffer. The column is then removed from the magnet, 5 mL sorting buffer is added, and the plunger is pushed through to elute purified CD4+ target cells.

The MBCS-0008 kit delivers high-purity enrichment and isolation of CD4+ T cells from PBMCs, as validated by flow cytometry using CD4-PE (Clone RPA-T4), CD3-APC, CD8a-FITC staining. The high-specificity anti-human CD4 monoclonal antibody ensures efficient capture with minimal non-specific carryover. Purity typically reaches 90–99%.

The MBCS-0008 kit is optimized for human PBMCs. Isolate PBMCs from human peripheral blood, pass through a 30 μm cell strainer, count cells, and centrifuge 1×10⁷ cells at 1500 rpm for 5 min. The kit is compatible with PBMCs from healthy donors and patients with various conditions.

Step 1: Sample Preparation. Isolate PBMCs from human peripheral blood, filter through a 30 μm cell strainer, count cells, and centrifuge 1×10⁷ cells at 1500 rpm for 5 min.
Step 2: Magnetic Labeling. Resuspend the cell pellet in 100 μL sorting buffer, add 20 μL CD4 magnetic beads, mix thoroughly, and incubate at 2–8°C for 15 min.
Step 3: Magnetic Separation. Load the sample onto a pre-rinsed magnetic column, collect the flow-through, and wash the column three times with 3 mL sorting buffer.
Step 4: Elution. Remove the column from the magnet, add 5 mL sorting buffer, immediately push the plunger through, and collect the eluted CD4+ target cells.
Total time: ~30–40 min.

Isolated CD4+ T cells are immediately ready for: immunology research, flow cytometry, cell culture and expansion, inflammation studies, infectious disease research, autoimmune disease research, drug discovery, genomic analysis (RNA-seq, scRNA-seq), and cancer immunotherapy. The gentle sorting protocol preserves cell viability and functional integrity.

The high-specificity anti-human CD4 monoclonal antibody ensures efficient capture of CD4+ T cells with minimal off-target binding. This results in high-purity enriched populations while preserving native surface markers, viability, and functional integrity — critical for downstream functional assays and sensitive single-cell analyses.

The biodegradable nanoscale matrix (50–100 nm) minimizes steric hindrance and mechanical stress on cells compared to larger microbeads. This ensures efficient antibody-target binding while preserving native cell surface markers, viability, and functional integrity. Additionally, the nanoscale bead size prevents column clogging, ensuring smooth and consistent separation performance.

We recommend flow cytometry using CD4-PE (Clone RPA-T4), CD3-APC, CD8a-FITC antibodies. Typical results show 90–99% CD4+ cells (CD4+ CD3+ CD8a−) in the positively selected fraction. Viability can be assessed using Trypan blue exclusion or 7-AAD staining. For T helper subset analysis, additional markers such as CD45RA vs. CD45RO, CXCR3, and CCR6 can be used.

Absolutely. Isolated CD4+ T cells are immediately ready for single-cell RNA sequencing (scRNA-seq) without additional washing. The gentle 50–100 nm nanobeads and streamlined MACS workflow preserve transcriptomic integrity, making the MBCS-0008 kit compatible with 10x Genomics and other major scRNA-seq platforms.

The MBCS-0008 Human CD4 Sorting Magnetic Beads should be stored at 2–8°C and used within 12 months of opening. Do not freeze. The streamlined MACS workflow with established protocols ensures consistent results across experiments.