Human CD8 Positive Selection Nanobead Kit CS-0023 | Cell Sorts™
CS-0023

Human CD8+ Nanobeads Sorting Kit

Column-Based Positive Human

Cell Sorts™ Human CD8+ Nanobeads Sorting Kit (CS-0023) enriches CD8-expressing cells from human PBMCs by column-based positive selection. Antibody-conjugated nanobeads directly label CD8+ cells, which are retained in a magnetic column while unlabeled cells pass through. Removing the column from the separator and flushing it with buffer recovers the labeled target-enriched fraction for research use.

For each 1×107 total cells, the documented labeling scale uses 80 µL pre-cooled separation buffer and 20 µL nanobeads, followed by a 15-minute incubation at 4°C and two 300 × g, 10-minute centrifugations. The supplied plots show representative post-selection CD8+ enrichment with recovery and viability distributions, but do not state a guaranteed percentage. Confirm identity, purity, viability, and recovery with a laboratory-validated assay before downstream studies.

Sorting Purity
Representative high CD8+ enrichment; exact specification not stated
Sorting Time
At least 35 min of documented timed steps plus column handling

Product Features of the Human CD8+ Nanobeads Sorting Kit

CS-0023 uses a documentation-aligned column-based positive-selection workflow to enrich a directly bead-labeled human CD8+ cell fraction from PBMCs using defined reagent dosing, controlled-temperature labeling, and capacity-matched magnetic columns.

Direct CD8 positive selection - Antibody-conjugated nanobeads label CD8-expressing cells for magnetic-column retention and elution
Defined labeling scale - Use 80 µL pre-cooled separation buffer and 20 µL nanobeads per 1×10⁷ total cells
Column-based capture and recovery - Unlabeled cells pass through while labeled CD8+ cells are retained, washed, and recovered after removing the column from the magnet
PBMC-focused workflow - Begin with density-gradient PBMCs, wash, perform red-blood-cell lysis as needed, and count cells before labeling
Scalable column capacities - Small columns support up to 1×10⁷ labeled or 1×10⁸ total cells; medium columns support up to 1×10⁸ labeled or 1×10⁹ total cells
Documented kit scale and storage - Up to 100 separations or 1×10⁹ total cells; store at 2-8°C protected from light and do not freeze

CS-0023 uses column-based positive selection to recover a bead-labeled human CD8+ cell fraction from PBMCs. The supplied document does not state a validated total processing time; two 10-minute centrifugations and one 15-minute labeling incubation total at least 35 minutes, with column preparation, loading, washing, elution, transfers, counting, and QC additional.

How the Human CD8+ Nanobeads Sorting Kit Works

Four stages summarizing the documented human PBMC CD8 positive-selection column workflow

1
Prepare and count human PBMCs for CS-0023 CD8 positive selection

Step 1: Prepare and Count PBMCs

Prepare human PBMCs by density-gradient separation, wash, perform red-blood-cell lysis as needed, and count the cells.

2
Directly label CD8-expressing cells with CS-0023 antibody-conjugated nanobeads

Step 2: Label CD8+ Cells

Centrifuge at 300 × g for 10 minutes. Resuspend 1×10⁷ total cells in 80 µL pre-cooled separation buffer, add 20 µL Human CD8 nanobeads, mix, and incubate for 15 minutes at 4°C.

3
Wash labeled cells and prepare a bubble-free magnetic separation column

Step 3: Wash and Prepare the Column

Add 1-2 mL buffer, centrifuge at 300 × g for 10 minutes, and resuspend in 500 µL buffer without exceeding 1×10⁸ cells/mL. Bring column buffer to room temperature, avoid bubbles, and pre-rinse with 500 µL for a small column or 3 mL for a medium column.

4
Retain, wash, and elute the bead-labeled CD8-enriched fraction from the column

Step 4: Separate, Wash, and Elute

Load the magnetized column, wash three times with 500 µL per wash for a small column or 3 mL per wash for a medium column, remove the column from the magnet, and flush the retained bead-labeled CD8+ fraction with 1 mL or 5 mL buffer, respectively.

Applications for the Human CD8+ Nanobeads Sorting Kit

Potential downstream uses for the directly bead-labeled CD8-enriched fraction are listed below. Confirm CD3/CD8 identity, purity, viability, recovery, residual populations, direct-label compatibility, and assay-specific suitability.

Flow-Cytometric Quality Control - Confirm CD8 enrichment, viability, recovery, and residual populations with a laboratory-validated panel CD8-Cell Immunophenotyping - Characterize CD3, CD8, CD4, and additional markers appropriate to the research question Cytotoxic T-Cell Activation and Functional Research - Use qualified CD8-enriched fractions in controlled stimulation and functional assays Cytokine and Signaling Research - Evaluate responses to defined stimuli after confirming post-sort identity and sample quality CD8 Subset Research - Profile naïve, memory, effector, or exhaustion-associated phenotypes with additional laboratory-selected markers Infectious-Disease and Vaccine Research - Study CD8-cell responses in fit-for-purpose non-clinical models Tumor Immunology Research - Investigate CD8-associated immune responses in appropriately controlled non-clinical assays Culture, Molecular, and Single-Cell Workflows - Proceed after verifying purity, viability, recovery, direct-label compatibility, and platform acceptance

The listed applications are potential research uses, not guaranteed performance claims. Confirm CD3/CD8 identity, purity, viability, recovery, direct-label compatibility, and functional suitability for the intended assay.

Kit Components

CS-0023 includes the documented Human CD8 nanobead reagent and user instructions. Separation buffer, capacity-matched single-use columns, a compatible column magnet, PBMC-preparation materials, centrifugation and counting equipment, collection tubes, and post-sort QC reagents are required separately.

  • Human CD8 nanobeads
  • User manual

Storage & Shelf Life

Storage
2-8°C protected from light; do not freeze; avoid high-speed centrifugation
Shelf Life
Not stated in supplied documentation

Human CD8+ Nanobeads Sorting Kit — Frequently Asked Questions

Everything you need to know about the CS-0023 Human CD8+ Nanobeads Sorting Kit

Click each question to expand the answer

CS-0023 enriches CD8-expressing human cells from PBMC preparations by direct positive selection. CD8 can also be detected on a subset of NK cells, so laboratories should verify CD3/CD8 identity and residual non-T-cell populations rather than assuming that every recovered CD8+ event is a cytotoxic T cell.
The kit uses column-based positive selection. Antibody-conjugated nanobeads directly label CD8+ cells, which are retained on a magnetized separation column while unlabeled cells pass through. The column is then removed from the magnet and the retained fraction is flushed into a collection tube.
Yes. CS-0023 is a positive-selection workflow, so the recovered target-enriched fraction has been directly contacted and labeled by the CD8 nanobead reagent. The supplied documentation does not describe a validated bead-release step; applications that require an unlabeled target fraction should be qualified separately.
The documented starting material is a human PBMC suspension prepared by density-gradient separation, followed by washing and red-blood-cell lysis as needed. Count the cells before labeling and confirm that the suspension is suitable for column loading.
For 1×107 total cells, resuspend the pellet in 80 µL pre-cooled separation buffer and add 20 µL Human CD8 nanobeads. For lower inputs, retain these minimum volumes; for higher inputs, scale buffer and reagent volumes proportionally.
The supplied document does not state a validated total processing time. Its listed timed steps include an initial 300 × g centrifugation for 10 minutes, a 15-minute labeling incubation at 4°C, and a second 300 × g centrifugation for 10 minutes. Column rinsing, loading, three washes, elution, transfers, counting, and QC require additional time.
A small column supports up to 1×107 labeled cells or 1×108 total cells; a medium column supports up to 1×108 labeled cells or 1×109 total cells. Pre-rinse with 500 µL or 3 mL buffer, respectively, then wash three times with the same per-wash volume.
Perform bead labeling at 2-8°C with pre-cooled separation buffer; the document cautions against incubating directly in ice. Bring buffer used for column separation to room temperature, avoid introducing bubbles, do not allow the column to run dry, and use each column only once.
The supplied document includes representative before-and-after flow plots showing strong CD8+ enrichment, together with recovery and viability distributions. It does not state an exact guaranteed purity, recovery, or viability specification. Donor variation, starting composition, aggregation, labeling, column loading, and gating can affect results.
Store the nanobead reagent at 2-8°C protected from light. Do not freeze it and avoid high-speed centrifugation. The documented kit scale is up to 100 separations or 1×109 total cells. A shelf-life duration is not stated in the supplied documentation.

Related topics covered:

column-based positive selection CD8+ T Cell isolation CD8+ T Cell purity verification CD8+ T Cell sorting protocol column-based vs column-free single-cell analysis (scRNA-seq) CD8+ T Cell isolation markers PBMC CD8+ T Cell sorting

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