CS-0001

Human B Cell Sorting Kit

Column-Free Negative Human

Cell Sorts™ Human B Cell Sorting Kit (CS-0001) enriches untouched CD19+ B cells from human PBMCs by column-free negative selection. An Fc receptor blocker helps reduce nonspecific binding, while a biotin-conjugated isolation cocktail labels non-B cells. Streptavidin nanobeads then magnetically mark the labeled non-target fraction. During tube-based magnetic separation, bead-bound cells are retained at the tube wall and the unlabeled B-cell suspension is transferred to a fresh tube without direct antibody or bead labeling of the target cells.

Supplied technical documentation specifies a 10-minute room-temperature cocktail incubation, a 5-minute room-temperature nanobead incubation, and a 3- or 5-minute magnetic hold, giving a documented 18–20 minute core separation after PBMC preparation and counting. One representative flow-cytometry example increased CD19+ cells from 4.72% before separation to 97.4% after sorting, and activated isolated cells retained proliferative capacity. Formats support up to 1×10⁸, 5×10⁸, or 1×10⁹ total starting cells. For research use only.

Sorting Purity

Representative result 97.4%

Sorting Time

18–20 min

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Product Features of the Human B Cell Sorting Kit

CS-0001 uses a documented column-free negative-selection workflow to enrich untouched human CD19+ B cells from density-gradient-prepared PBMCs.

Column-free negative selection — Magnetically labeled non-B cells are retained at the tube wall while the enriched target fraction is transferred to a fresh vessel

Untouched CD19+ target fraction — Recovered B cells are not directly labeled with antibodies or magnetic nanobeads

Representative 97.4% post-sort purity — A documented example result, not a guaranteed value for every donor or run

18–20 minute documented core separation — 10-minute cocktail labeling, 5-minute nanobead binding, and a 3–5-minute magnetic hold

Scalable kit formats — Options support up to 1×10⁸, 5×10⁸, or 1×10⁹ total starting cells

Defined tube-based workflow — 2.5 mL or 7.5 mL working volumes match compatible 5 mL or 15 mL magnetic separation formats

CS-0001 uses column-free negative selection to enrich untouched CD19+ B cells from human PBMCs. The documented 18–20 minute core separation comprises 10-minute cocktail labeling, 5-minute nanobead binding, and a 3–5-minute magnetic hold; PBMC preparation, counting, optional post-collection centrifugation, and QC are additional.

How the Human B Cell Sorting Kit Works

Simple 4-step protocol for rapid cell isolation

Step 1: Prepare and Count PBMCs

Prepare human PBMCs by density-gradient separation, count viable cells, and resuspend at no more than 1×10⁸ cells/mL. For 1×10⁷ cells, use a 100 µL starting suspension.

Step 2: Block Fc Receptors and Label Non-B Cells

Add 10 µL Human FcR Blocker and 10 µL Human B Cell Isolation Cocktail per 1×10⁷ cells. Mix gently and incubate for 10 minutes at room temperature.

Step 3: Bind Streptavidin Nanobeads

Vortex the nanobeads for 5–30 seconds, add 10 µL per 1×10⁷ cells, mix, and incubate for 5 minutes at room temperature. Remix at about 2.5 minutes if cells settle.

Step 4: Magnetically Deplete and Collect B Cells

Bring the sample to 2.5 mL in a 5 mL tube or 7.5 mL in a 15 mL tube. Magnetize for 3 minutes on a single-position separator or 5 minutes on a multi-position separator, then transfer the untouched target-cell suspension to a fresh tube.

Applications for the Human B Cell Sorting Kit

This Human B Cell Sorting Kit supports a wide range of downstream applications for CD19+ B Cell isolation and column-free negative selection workflows from human PBMCs.

Flow Cytometric Immunophenotyping — Measure post-sort CD19+ purity, viability, and residual non-B-cell populations with a laboratory-validated panel B Cell Activation and Proliferation — Evaluate receptor-driven activation and proliferation after confirming post-sort cell quality Immunoglobulin Secretion Studies — Assess antibody secretion in appropriately controlled B-cell culture systems BCR Signaling Research — Investigate B-cell receptor signaling using an untouched target-cell fraction Cell Culture and Expansion — Establish downstream culture workflows after post-sort count, viability, and sterility assessment Transcriptomic and BCR Repertoire Analysis — Prepare input for RNA, BCR-sequencing, or single-cell workflows when platform-specific QC criteria are met Autoimmune, Infectious-Disease, and Vaccine Research — Study human B-cell responses in appropriately controlled research models Drug Discovery — Evaluate candidate immunomodulators in validated human B-cell assays

In addition, the Human B Cell Sorting Kit is compatible with a wide range of downstream applications, making it a versatile tool for your immunology research workflow.

Kit Components

Each Human B Cell Sorting Kit contains everything needed for high-purity column-free negative selection from human PBMCs.

Human FcR Blocker
Human B Cell Isolation Cocktail
Streptavidin Nanobeads

Storage & Shelf Life

Storage

Store refrigerated at 4°C; do not freeze

Shelf Life

Refer to the product label

Human B Cell Sorting Kit — Frequently Asked Questions

Everything you need to know about the CS-0001 Human B Cell Sorting Kit

Click each question to expand the answer

CS-0001 is documented for enriching untouched CD19+ B cells from human PBMCs. Prepare PBMCs by density-gradient separation, count viable cells, and resuspend them in FACS buffer before labeling. Red-blood-cell lysis is not recommended in the supplied workflow.

An Fc receptor blocker helps reduce nonspecific binding, and a biotin-conjugated antibody cocktail labels non-B cells. Streptavidin nanobeads bind the labeled non-target fraction. A tube magnet retains those bead-bound cells, while the unlabeled CD19+ B-cell suspension is transferred to a fresh tube.

In one representative flow-cytometry example supplied with the product documentation, CD19+ cells increased from 4.72% before separation to 97.4% after sorting. This is an example result rather than a guarantee. Donor biology, PBMC quality, starting composition, cell concentration, mixing, incubation accuracy, magnet performance, and operator technique can affect purity and recovery.

The core separation comprises a 10-minute room-temperature cocktail incubation, a 5-minute room-temperature nanobead incubation, and a 3-minute single-position or 5-minute multi-position magnetic hold. PBMC preparation, cell counting, reagent setup, optional post-collection centrifugation, and quality-control testing require additional time.

For each 1×10⁷ cells in 100 µL, the documented starting point is 10 µL Human FcR Blocker, 10 µL Human B Cell Isolation Cocktail, and 10 µL Streptavidin Nanobeads. Scale all volumes proportionally, keep the suspension at no more than 1×10⁸ cells/mL, and use the 1×10⁷-cell calculation for smaller inputs unless your laboratory has validated another scale.

The separation workflow specifies FACS buffer consisting of 1× PBS, 2% FBS, and 0.02% EDTA. Reagents are stored refrigerated at 4°C and must not be frozen. The supplied blocker and cocktail are formulated in PBS containing 0.1% BSA; follow the product label for expiry and lot-specific instructions.

The documentation describes a sterile flow tube with a compatible single-position magnet, a 15 mL tube with a compatible multi-position magnet, or a 50 mL tube with a compatible single-position magnet. Typical working volumes are 2.5 mL for a 5 mL tube or 7.5 mL for a 15 mL tube. Use only combinations validated for your separator.

No. The depletion reagents label non-B cells, so the recovered target fraction is not directly tagged with the isolation antibodies or magnetic nanobeads. Supplied documentation also shows a representative activation experiment in which isolated cells retained proliferative capacity; laboratories should still confirm viability and function for their own downstream assay.

Potential uses include flow-cytometric immunophenotyping, activation and proliferation assays, immunoglobulin-secretion studies, BCR signaling, cell culture, transcriptomic or BCR-repertoire analysis, disease-response research, and drug discovery. Confirm post-sort count, viability, purity, and any platform-specific input requirements before use.

First check PBMC quality, viability, clumping, starting cell concentration, and proportional reagent scaling. Vortex nanobeads for 5–30 seconds before use, mix gently but thoroughly, and remix the sample around halfway through the 5-minute bead incubation if cells settle. Verify the final working volume, magnet compatibility, full 3- or 5-minute magnetic hold, and clean transfer of the target suspension without disturbing retained cells.