Human CD8+ T Cell Sorting Kit CS-0007 for column-free T cell isolation | Cell Sorts™
CS-0007

Human CD8+ T Cell Sorting Kit

Column-Free Negative Human

Cell Sorts™ Human CD8+ T Cell Sorting Kit (CS-0007) enriches untouched CD8+ T cells from human PBMCs through column-free negative selection. A biotinylated antibody cocktail identifies non-CD8 cells, which are subsequently bound by streptavidin magnetic nanobeads. In a compatible tube separator, labeled cells are immobilized against the tube wall and the CD8+ T cell-enriched suspension is transferred to a clean collection tube.

The documented core workflow takes 18–20 minutes and delivers purity above 90%, with results up to 98% under tested conditions. Available formats support up to 1×10⁸, 5×10⁸, or 1×10⁹ total starting cells. Actual performance varies with donor composition, PBMC quality, cell concentration, handling, and post-isolation QC. For research use only.

Sorting Purity
Above 90%, up to 98%
Sorting Time
18–20 min

Product Features of the Human CD8+ T Cell Sorting Kit

The Human CD8+ T Cell Sorting Kit delivers industry-leading performance for rapid CD8+ T Cell isolation from PBMCs via column-free negative selection.

Column-free negative selection — Non-target cells are magnetically retained in a compatible tube separator; no separation column is used
Untouched CD8+ T-cell fraction — Target cells are not directly labeled with antibodies or nanobeads
Documented purity above 90%, up to 98% — Actual results depend on donor sample quality, input composition, and workflow execution
18–20 minute core workflow — Covers cocktail labeling, nanobead binding, magnetic separation, and collection after PBMC preparation
Defined reagent ratio — 100 µL isolation cocktail and 100 µL nanobeads per mL of cells at up to 1×10⁸ cells/mL
Research-ready output — Supports flow cytometry, culture, cytotoxicity, activation, sequencing, and related CD8+ T-cell studies after QC

CS-0007 uses column-free negative selection to enrich untouched human CD8+ T cells from PBMCs. The documented core workflow takes approximately 18–20 minutes after PBMC preparation and counting, with purity reported above 90% and up to 98%.

How the Human CD8+ T Cell Sorting Kit Works

Simple 4-step protocol for rapid cell isolation

1
Step 1 of CD8+ T cells sorting: Sample Preparation - Prepare PBMC single-cell suspension with FcR Blocker for Human CD8+ T Cell Sorting Kit CS-0007

Step 1: Prepare the PBMC Suspension

Isolate PBMCs by density-gradient centrifugation, determine viable cell concentration, and prepare a uniform single-cell suspension. Resuspend at up to 1×10⁸ cells/mL; at least 1×10⁷ cells per separation is recommended.

2
Step 2 of CD8+ T cells sorting: Antibody Cocktail Labeling - Biotin-conjugated antibodies target non-CD8+ T cells for depletion with CS-0007

Step 2: Label Non-Target Cells

Add 100 µL of isolation cocktail per mL of cell suspension, mix thoroughly, and incubate for 10 minutes at room temperature.

3
Step 3 of CD8+ T cells sorting: Magnetic Nanobead Binding - Streptavidin nanobeads bind labeled non-target cells for magnetic removal with CS-0007

Step 3: Bind Magnetic Nanobeads

Add 100 µL of nanobeads per mL of cells, mix, and incubate for 5 minutes at room temperature. An optional wash may be appropriate for selected patient-derived PBMC samples.

4
Step 4 of CD8+ T cells sorting: Column-Free Separation - Purified CD8+ T cells collected by magnetic separator pour-off for CS-0007

Step 4: Separate and Recover CD8+ T Cells

Adjust to the specified final volume, place the tube in a compatible magnetic separator for the documented hold time, and transfer the enriched suspension while the tube remains in the magnet without disturbing retained cells.

Applications for the Human CD8+ T Cell Sorting Kit

This Human CD8+ T Cell Sorting Kit supports a wide range of downstream applications for CD8+ T Cell isolation and column-free negative selection workflows from human PBMCs.

Flow Cytometry — Enrich CD3+CD8+ T cells before subset, activation-marker, or viability analysis Cell Culture — Establish cultures from an untouched enriched fraction after confirming purity, viability, and sterility Activation & Proliferation — Evaluate CD8+ T-cell responses using a fit-for-purpose stimulation protocol Cytotoxicity & Degranulation — Use the enriched population as an effector-cell input after confirming assay requirements Cytokine Response — Measure stimulus-dependent cytokine production in an enriched CD8+ T-cell population Workflow Development — Optimize input concentration, magnetic hold, transfer technique, recovery, and donor consistency

In addition, the Human CD8+ T Cell Sorting Kit is compatible with a wide range of downstream applications, making it a versatile tool for your immunology research workflow.

Kit Components

Each Human CD8+ T Cell Sorting Kit contains everything needed for high-purity column-free negative selection from human PBMCs.

  • Human CD8+ T Cell Isolation Cocktail
  • Streptavidin Nanobeads
  • User Manual

Storage & Shelf Life

Storage
Store at 2-8°C protected from light
Shelf Life
12 months

Human CD8+ T Cell Sorting Kit — Frequently Asked Questions

Everything you need to know about the CS-0007 Human CD8+ T Cell Sorting Kit

Click each question to expand the answer

CS-0007 is designed to enrich untouched human CD8+ T cells from PBMC preparations. Starting-cell viability, aggregation, donor-to-donor variation, and the proportion of CD8+ T cells can affect purity and recovery.
A biotinylated cocktail and magnetic nanobeads label non-target cells. A compatible tube magnet retains those labeled cells, while unlabeled CD8+ T cells remain in suspension for transfer. No magnetic separation column is required.
No. The target CD8+ T-cell fraction is not directly labeled by the depletion reagents and is recovered as an untouched, bead-free population.
The supplied technical documentation reports purity above 90%, with results up to 98%. This is not a guaranteed value; each laboratory should verify purity, viability, recovery, and yield under its own sample and workflow conditions.
The documented core isolation workflow is approximately 18–20 minutes after PBMC preparation and counting. It includes a 10-minute cocktail incubation, a 5-minute nanobead incubation, magnetic separation, and collection.
For a suspension at up to 1×10⁸ cells/mL, the documentation specifies 100 µL of isolation cocktail and 100 µL of nanobeads per mL of cells. Scale volumes proportionally and follow the current product instructions.
The standard documented workflow proceeds from nanobead incubation to magnetic separation. An optional wash may be considered for selected patient-derived PBMC samples; validate any modification in your own laboratory.
A compatible magnetic tube separator, suitable tubes, routine PBMC-processing equipment, and tools for cell counting and downstream flow-cytometry QC are required. Confirm tube volume and separator compatibility before starting.
After confirming purity and viability, the enriched fraction may support flow cytometry, cell culture, activation and expansion, cytotoxicity studies, cytokine analysis, TCR studies, gene-editing research, and single-cell workflows.
CS-0007 uses a column-free tube-magnet workflow and has a documented 18–20 minute core process with purity reported above 90% and up to 98%. CS-0008 uses an L-column workflow, has documented purity of 80–93% under tested conditions, and does not have a validated total processing time stated in the supplied documentation.

Related topics covered:

column-free negative selection CD8+ T Cell isolation CD8+ T Cell purity verification CD8+ T Cell sorting protocol column-free isolation single-cell analysis (scRNA-seq) CD8+ T Cell isolation markers PBMC CD8+ T Cell sorting

Start Your Efficient & Precise Cell Sorting Journey

Contact our technical team for customized sorting solutions and product recommendations

Contact Us Now Download Product Catalog

Discover more from Cell Sorts™

Subscribe now to keep reading and get access to the full archive.

Continue reading