CS-0014

Human T Cell Sorting Kit

Column-Free Negative Human

Cell Sorts™ Human T Cell Sorting Kit (CS-0014) enriches an untouched pan T-cell fraction from prepared human peripheral blood mononuclear cells (PBMCs) by column-free negative selection. A human T-cell negative-selection antibody cocktail and matching magnetic beads label non-target nucleated cells for magnetic retention; the unretained CD3+ T-cell-enriched suspension is recovered by controlled pour-off.

For 1×10⁷ starting cells, the documented core workflow uses 10 µL antibody cocktail for 5 minutes at room temperature, 8 µL magnetic beads for 5 minutes, and a 3-minute magnetic hold after adding 2 mL separation buffer. One representative CD3-FITC result increased the monitored CD3+ fraction from 53.6% before separation to 96.8% afterward; this is not a guaranteed specification. Confirm identity, purity, viability, recovery, and functional suitability for every donor and downstream assay. For research use only.

Sorting Purity

Representative result 96.8%

Sorting Time

Approximately 13 min listed core steps after PBMC preparation

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Product Features of the Human T Cell Sorting Kit

CS-0014 uses a documented column-free negative-selection workflow to enrich an untouched human pan T-cell fraction from prepared PBMCs using 5-minute antibody labeling, 5-minute bead binding, and a 3-minute magnetic hold.

Column-free negative selection - Non-target nucleated cells are antibody- and bead-labeled for magnetic retention without a separation column

Untouched target fraction - The unretained CD3+ T-cell-enriched suspension is recovered by controlled pour-off rather than direct positive labeling

Defined room-temperature core steps - The documented method uses 5 minutes for antibody labeling, 5 minutes for bead binding, and 3 minutes on the magnet

Scale-defined for 1×10⁷ cells - Use 10 µL antibody cocktail, 8 µL magnetic beads, and 2 mL separation buffer; follow the current instructions when scaling

Flexible PBMC preparation - Guidance covers density-gradient peripheral-blood PBMCs, leukopak material, and DNase I handling for frozen PBMCs

Evidence-qualified performance - One representative CD3-FITC example increased from 53.6% to 96.8%; results are sample- and workflow-dependent

The Human T Cell Sorting Kit uses column-free negative selection to isolate untouched Untouched Human Pan T Cells (CD3+ Enriched Fraction) from human PBMCs. Review the product-specific four-stage workflow below. The stated sorting time of Approximately 13 min listed core steps after PBMC preparation covers the specified timed stages only; sample preparation, handling, optional collection, and quality control require additional time. Follow the current product instructions and validate each preparation before downstream use.

How the Human T Cell Sorting Kit Works

Four stages summarizing the documented PBMC pan T-cell negative-selection workflow

Step 1: Prepare and Count PBMCs

Prepare human PBMCs from EDTA-anticoagulated peripheral blood or leukopak material, wash and filter, then resuspend in pre-chilled separation buffer at no more than 5×10⁷ cells/mL. Follow the documented DNase I procedure for frozen PBMCs.

Step 2: Label Non-Target Cells

For 1×10⁷ prepared cells in a 5 mL tube, add 10 µL Human T Cell Negative Selection Antibody Cocktail, mix gently, and incubate for 5 minutes at room temperature.

Step 3: Bind Magnetic Beads

Add 8 µL thoroughly mixed magnetic beads, mix gently, incubate for 5 minutes at room temperature, then add 2 mL separation buffer and mix by pipetting.

$CS-0014 workflow step 4: magnetically retain labeled cells for 3 minutes and pour off the pan T-cell-enriched fraction$

Step 4: Capture and Recover Pan T Cells

Hold the tube in the specified compatible 5 mL magnetic separator for 3 minutes at room temperature, then lift and continuously invert the magnet, rack, and tube together to pour the untouched CD3+ T-cell-enriched suspension into a fresh tube.

Applications for the Human T Cell Sorting Kit

Potential downstream uses for the enriched untouched pan T-cell fraction are listed below. Confirm CD3+ identity, subset composition, purity, viability, recovery, and functional suitability under laboratory-specific conditions.

Flow Cytometric Quality Control - Assess CD3+ enrichment, viability, recovery, and residual populations with a laboratory-validated panel Pan T Cell Immunophenotyping - Characterize CD4+, CD8+, and other T-cell subsets after confirming post-sort identity and sample quality Activation and Proliferation Research - Use qualified pan T-cell preparations in controlled receptor-stimulation and expansion assays Cytokine Response Studies - Evaluate responses to defined stimuli after fit-for-purpose purity and viability checks Cell Culture and Process Development - Establish laboratory-validated culture, handling, and expansion workflows for research use Molecular and Single-Cell Profiling - Prepare DNA, RNA, protein, TCR-repertoire, or single-cell inputs when platform-specific acceptance criteria are met Gene-Editing and Transduction Feasibility Research - Evaluate research workflows without implying guaranteed editing, transduction, or functional outcomes Cell-Therapy Workflow Research - Support non-clinical process-development studies after assay-specific qualification; not for direct clinical use

The listed applications are potential research uses, not guaranteed performance claims. Confirm CD3+ identity, subset composition, purity, viability, recovery, and functional suitability under laboratory-specific conditions.

Kit Components

CS-0014 includes the documented Human T Cell Negative Selection Antibody Cocktail and Human T Cell Negative Selection Magnetic Beads. Separation buffer, 5 mL flow tubes, compatible magnetic equipment, PBMC-preparation materials, and post-sort QC reagents are required separately.

Human T Cell Negative Selection Antibody Cocktail
Human T Cell Negative Selection Magnetic Beads

Storage & Shelf Life

Storage

2-8°C; do not freeze

Shelf Life

Refer to the product label

Human T Cell Sorting Kit — Frequently Asked Questions

Everything you need to know about the CS-0014 Human T Cell Sorting Kit

Click each question to expand the answer

CS-0014 enriches an untouched human pan T-cell fraction from prepared human PBMCs. The supplied representative quality-control example monitors CD3 expression by flow cytometry; the recovered fraction may contain multiple T-cell subsets, so laboratories should define their own post-sort identity panel.

The kit uses column-free negative selection. An antibody cocktail and magnetic beads label non-target nucleated cells for magnetic retention, while the unretained CD3+ T-cell-enriched suspension is recovered by controlled pour-off.

The supplied procedure covers density-gradient-prepared PBMCs from EDTA-anticoagulated peripheral blood and prepared leukopak material. For frozen PBMCs, it specifies room-temperature DNase I treatment at a final concentration of 100 µg/mL for at least 15 minutes, followed by washing, 37 µm filtration, counting, and resuspension at no more than 5×10⁷ cells/mL.

Place 1×10⁷ prepared cells in a 5 mL flow tube, add 10 µL Human T Cell Negative Selection Antibody Cocktail for 5 minutes at room temperature, then add 8 µL thoroughly mixed magnetic beads for a further 5 minutes. Add 2 mL separation buffer before magnetic separation.

The separation buffer is PBS at pH 7.2 containing 0.5% BSA and 2 mM EDTA. The procedure notes that HSA, human serum, or FBS may replace BSA and sodium citrate may replace EDTA. Keep cells cold, use pre-chilled buffer, work promptly, and perform the two listed labeling incubations at room temperature.

The listed quantities are for 1×10⁷ total cells. For fewer cells, the procedure instructs users to retain the 1×10⁷-cell reagent amounts; for more cells, scale reagent volumes proportionally. The documented capacity is up to 1×10⁹ total cells. Follow the current product instructions and validate mixing, tube, and magnet capacity at scale.

After adding 2 mL separation buffer and mixing by pipetting, place the 5 mL tube in the specified compatible magnetic separator for 3 minutes at room temperature. Lift the magnet and continuously invert the magnet, rack, and tube together to pour the T-cell-enriched suspension into a fresh tube without disturbing retained cells.

In one supplied CD3-FITC flow-cytometry example, the monitored CD3+ fraction increased from 53.6% before separation to 96.8% afterward. This is a representative result, not a guaranteed specification. Donor composition, PBMC preparation, cell condition, reagent handling, magnetic equipment, and gating strategy can affect the outcome.

The documented kit components are the Human T Cell Negative Selection Antibody Cocktail and Human T Cell Negative Selection Magnetic Beads. The laboratory must supply separation buffer, 5 mL flow tubes, a compatible magnetic separator, PBMC-preparation materials, counting equipment, and reagents for post-sort quality control.

Store the supplied components at 2-8°C and do not freeze; refer to the product label for expiry. CS-0014 is for research use only and is not intended for direct clinical therapy. Confirm identity, purity, viability, recovery, and functional suitability for every donor, preparation method, and downstream assay.

Human T Cell Sorting Kit

Product Features of the Human T Cell Sorting Kit

How the Human T Cell Sorting Kit Works

Step 1: Prepare and Count PBMCs

Step 2: Label Non-Target Cells

Step 3: Bind Magnetic Beads

Step 4: Capture and Recover Pan T Cells

Applications for the Human T Cell Sorting Kit

Kit Components

Storage & Shelf Life

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Human T Cell Sorting Kit — Frequently Asked Questions

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