Cell Sorts Mouse CD8+ Cell Sorting Kit CS-0034 product showing column-free positive selection with Releasable Beads for isolating CD8+ cytotoxic T cells from mouse splenocytes with >95% purity
CS-0034

Mouse CD8+ Cell Sorting Kit

Column-Free Positive Mouse

The Cell Sorts Mouse CD8+ Cell Sorting Kit (CS-0034) uses column-free positive selection with Releasable Beads to capture CD8+ cytotoxic T lymphocytes from mouse splenocytes and other lymphoid tissue single-cell suspensions with >95% purity and ~35–40 min hands-on time. CD8a antibody-conjugated nanobeads label target CTLs; a magnetic stand captures bead-bound cells while non-target cells are washed away. Release Buffer dissociates beads for bead-free, untagged CD8+ cells.

Broadly compatible with mouse splenocytes and lymphoid tissue single-cell suspensions. Validated on C57BL/6. No separation column needed — only a magnetic stand. Released cells carry no magnetic bead residue, preserving native surface markers and full cytotoxic killing capacity. Bead-free CD8+ CTLs are immediately ready for tumor cell killing assays, viral immunity studies, CAR-T development, scRNA-seq, and adoptive CTL transfer.

Sorting Purity
Up to 95.1%
Sorting Time
~35-40 min

Product Features of the Mouse CD8+ Cell Sorting Kit

The Mouse CD8+ Cell Sorting Kit delivers industry-leading performance for rapid CD8+ T Cell isolation from PBMCs via column-free positive selection.

Releasable nanobead technology — Capture CD8+ cells with nanobeads, then release beads from cell surface for bead-free, untagged target cells
Purity >95% — High-efficiency positive selection for ultra-pure CD8+ cytotoxic T cell populations
Broad sample compatibility — Directly capture target cells from mouse splenocytes and other lymphoid tissue single-cell suspensions
Bead-free output — Released cells carry no magnetic bead residue, ideal for downstream functional assays and cell culture
Optimized for mouse immune cells — Specifically validated on C57BL/6 mouse splenocytes

The Mouse CD8+ Cell Sorting Kit uses column-free positive selection to isolate purified CD8+ T Cells from human PBMCs. To achieve these results, follow this simple 4-step protocol that takes just ~35-40 min from start to finish. No centrifugation and no lengthy incubations. Furthermore, this streamlined protocol ensures consistent results across experiments.

How the Mouse CD8+ Cell Sorting Kit Works

Simple 4-step protocol for rapid cell isolation

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Step 1: Sample Preparation - Prepare single-cell suspension for magnetic cell sorting

Step 1: Sample Preparation

Grind mouse spleen through a 70 μm cell strainer, perform ACK lysis, wash, filter, count cells, and resuspend in separation buffer at 1×10⁸ cells/mL.

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Step 2: Antibody Cocktail Incubation - Label non-target cells with biotin-conjugated antibodies

Step 2: Antibody Labeling & Magnetic Bead Capture

Aliquot 500 μL cell suspension (5×10⁷ cells), add 10 μL CD8 Capture Antibody, mix well, 4°C 10 min. Then add 100 μL pre-washed Releasable Beads, mix well, 4°C 10 min to capture target CD8⁺ cells.

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Step 3: Magnetic Nanobead Binding - Streptavidin nanobeads bind to labeled non-target cells

Step 3: Magnetic Enrichment & Wash

Dilute to 2.5 mL with separation buffer, magnetic stand 5 min, aspirate and discard supernatant. Wash captured cells three times with 2 mL buffer (5 min magnetic capture each) to remove non-target cells.

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Step 4: Column Separation - Purified target cells collected via column or magnetic separator

Step 4: Bead Release & Cell Collection

Resuspend in 1 mL Release Buffer, transfer to 1.5 mL tube, RT rotation 10 min. Gently pipette repeatedly, magnetic stand 5 min, collect bead-free CD8⁺ supernatant. Repeat once, pool both eluates, centrifuge 500×g 5 min.

Applications for the Mouse CD8+ Cell Sorting Kit

This Mouse CD8+ Cell Sorting Kit supports a wide range of downstream applications for CD8+ T Cell isolation and column-free positive selection workflows from mouse splenocytes or lymph node cells.

Cytotoxic T Cell Research — CTL-mediated cytotoxicity, cytokine production, and effector function studies in mouse models Cancer Immunology — Tumor-infiltrating CD8+ T cell studies, immune checkpoint inhibitor evaluation, and anti-tumor response analysis Infectious Disease Research — Virus-specific CD8+ T cell response and protective immunity studies in mouse infection models Autoimmune Disease Models — CD8+ T cell-mediated autoimmunity mechanism research Flow Cytometry — High-purity CD8+ cell populations ready for FITC anti-mouse CD8 (Clone 53-5.8) staining and analysis Cell Culture — Bead-free, functionally intact CD8+ T cells ideal for in vitro expansion and manipulation Molecular Biology — Purified CD8+ cells for RNA/DNA extraction and gene expression analysis

In addition, the Mouse CD8+ Cell Sorting Kit is compatible with a wide range of downstream applications, making it a versatile tool for your immunology research workflow.

Kit Components

Each Mouse CD8+ Cell Sorting Kit contains everything needed for high-purity column-free positive selection from human PBMCs.

  • CD8 Capture Antibody
  • Releasable Beads
  • Beads Release Buffer
  • User manual

Storage & Shelf Life

Storage
Store at 2-8°C protected from light
Shelf Life
12 months

Mouse CD8+ Cell Sorting Kit — Frequently Asked Questions

Everything you need to know about the CS-0034 Mouse CD8+ Cell Sorting Kit

Click each question to expand the answer

The CS-0034 Mouse CD8+ Cell Sorting Kit from Cell Sorts uses column-free positive selection with Releasable Beads. CD8a antibody-conjugated nanobeads directly bind CD8+ cytotoxic T cells; a magnetic stand captures bead-bound target cells while unlabeled non-target cells remain in suspension. Release Buffer then dissociates beads from the cell surface — yielding bead-free, untagged CD8+ T cells ready for downstream functional assays and cell culture.
Purity of isolated mouse CD8+ T cells typically reaches >95%, as validated by flow cytometry using anti-mouse CD8a antibody staining. High-efficiency positive selection with Releasable Beads enriches ultra-pure CD8+ cytotoxic T cell populations from mouse splenocytes and other lymphoid tissue single-cell suspensions while depleting B cells, helper T cells, NK cells, and myeloid cells.
The CS-0034 kit is optimized for mouse splenocytes and lymph node cell suspensions. Prepare a single-cell suspension by gentle mechanical disruption through a 70 μm cell strainer. Perform red blood cell lysis if needed (e.g., ACK lysis buffer). The kit is compatible with C57BL/6, BALB/c, and other common mouse strains used in tumor immunology and viral immunity research.
Step 1: Sample Preparation. Grind mouse spleen through a 70 μm cell strainer, perform ACK lysis, wash, filter, count cells, and resuspend in separation buffer at 1×10⁸ cells/mL.
Step 2: Antibody Labeling & Magnetic Bead Capture. Aliquot 500 μL cell suspension (5×10⁷ cells), add 10 μL CD8 Capture Antibody, mix well, 4°C 10 min. Then add 100 μL pre-washed Releasable Beads, mix well, 4°C 10 min to capture target CD8⁺ cells.
Step 3: Magnetic Enrichment & Wash. Dilute to 2.5 mL with separation buffer, magnetic stand 5 min, aspirate and discard supernatant. Wash captured cells three times with 2 mL buffer (5 min magnetic capture each) to remove non-target cells.
Step 4: Bead Release & Cell Collection. Resuspend in 1 mL Release Buffer, transfer to 1.5 mL tube, RT rotation 10 min. Gently pipette repeatedly, magnetic stand 5 min, collect bead-free CD8⁺ supernatant. Repeat once, pool both eluates, centrifuge 500×g 5 min.
Total hands-on time: ~35–40 min; total protocol: ~1.5–2 hours.
Isolated mouse CD8+ cytotoxic T cells are immediately ready for: cytotoxicity assays (tumor cell killing, chromium release), viral immunity studies (influenza, LCMV, HIV model), tumor immunology research (TIL analysis, neoantigen recognition), CAR-T cell therapy development in mouse models, adoptive T cell transfer experiments, CRISPR gene editing, single-cell RNA sequencing (scRNA-seq), and TCR repertoire sequencing.
The CS-0034 column-free approach eliminates column equilibration, loading, and washing steps. No column infrastructure is needed, making it ideal for parallel processing of multiple samples. Purity (>95%) and recovery are comparable to column-based positive selection, with the added convenience of Releasable Beads for downstream flexibility.
We recommend flow cytometry using anti-mouse CD8a-APC (Clone 53-6.7), CD3-FITC, CD4-PerCP-Cy5.5, and B220-PE antibodies. Typical results show >95% CD8+CD3+ cells with minimal CD4+ or B220+ contamination. For functional validation, perform cytotoxicity assays by co-culturing isolated CTLs with target tumor cells (e.g., EL4, B16) and measuring killing efficiency by chromium release or flow cytometry-based apoptosis detection.
Absolutely. Since isolated mouse CD8+ T cells are bead-free after Release Buffer treatment, they are immediately compatible with single-cell RNA sequencing (scRNA-seq), TCR repertoire sequencing, and other single-cell technologies. This enables high-resolution profiling of cytotoxic T cell heterogeneity, clonality analysis, and exhaustion marker profiling (PD-1, TIM-3, LAG-3) at single-cell resolution. Cell Sorts protocols are validated for 10x Genomics and other major scRNA-seq platforms.
The CS-0034 kit targets mouse CD8+ cytotoxic T cells using CD8a single-marker positive selection with Releasable Beads, enriching CTLs for tumor killing and viral immunity studies with >95% purity. The CS-0028 kit targets mouse CD4+ helper T cells using CD4 single-marker positive selection, enriching Th cells for cytokine and autoimmune studies. Both use column-free positive selection with releasable nanobead technology. Together they enable comprehensive mouse T cell subset analysis.
Yes, the CS-0034 kit is suitable for TIL CD8+ T cell enrichment. After dissociating mouse tumor tissue into a single-cell suspension (e.g., using collagenase/DNase digestion), the column-free positive selection protocol with Releasable Beads efficiently enriches CD8+ cytotoxic T cells from the heterogeneous TIL population. Bead-free cells are ready for cytotoxicity assays, TCR sequencing, or adoptive transfer experiments.

Related topics covered:

column-free positive selection CD8+ T Cell isolation CD8+ T Cell purity verification CD8+ T Cell sorting protocol column-free isolation single-cell analysis (scRNA-seq) CD8+ T Cell isolation markers PBMC CD8+ T Cell sorting

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