How do I verify CD56+ NK cell purity after sorting with the MBCS-0009 kit?

We recommend flow cytometry using CD56-PE (Clone B159), CD3-APC, CD16-FITC antibodies. Typical results show 90–99% CD56+ cells in the positively selected fraction, with minimal CD3+ T cell contamination. Viability can be assessed using Trypan blue exclusion or 7-AAD staining. Since nanobeads do not need to be removed post-separation, no additional washing is required before staining.

MBCS-0009

Human CD56 Sorting Magnetic Beads

CD Beads Fresh or frozen human PBMCs, Leukapheresis products, Single-cell suspensions from various tissues

The Cell Sorts MBCS-0009 Human CD56 Sorting Magnetic Beads uses column-based positive selection to enrich and purify CD56+ NK cells from human PBMCs with high purity in ~30–40 min. Monoclonal anti-CD56 mouse IgG1 antibody-conjugated nanobeads (50–100 nm) specifically label CD56+ NK cells, which are retained on a pre-rinsed magnetic column while unlabeled non-target cells flow through. Eluted cells are immediately ready for flow cytometry, cell culture, NK cell functional assays, and cancer immunotherapy research. No bead removal is required post-separation.

10 μL nanobeads per 1×10⁷ MNCs, incubated at 2–8°C for 15 min, delivers high purity and viability CD56+ NK cell populations. The streamlined MACS workflow with established protocols ensures consistent results. The beads are compatible with gM/gL columns and magnets, and the HSA plus Poloxamer 188 stabilized formulation ensures optimal performance across experiments.

Product Specifications

Particle Size

13 nm

Sorting Method

Column-Based

Sorting Principle

Positive

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Core Advantages of the Human CD56 Sorting Magnetic Beads

The Human CD56 Sorting Magnetic Beads delivers industry-leading performance for rapid CD56+ NK Cell enrichment from Fresh or frozen human PBMCs, Leukapheresis products, Single-cell suspensions from various tissues samples via column-based positive selection.

Monoclonal anti-human CD56 antibody (mouse IgG1) — ensures high specificity and binding affinity for CD56+ NK cells

No bead removal required post-separation — cells are immediately ready for downstream use

Compatible with gM/gL columns and magnets — as well as other compatible column/magnet systems

High purity and viability — of enriched CD56+ NK cell populations

Scalable protocol — 10 μL nanobeads per 10⁷ total MNCs for optimal enrichment

Gentle incubation conditions — 15 min at 2–8°C preserves cell function and surface markers

HSA plus Poloxamer 188 stabilized formulation — ensures optimal performance and consistency

The Human CD56 Sorting Magnetic Beads uses column-based positive selection to enrich purified CD56+ NK Cells from Fresh or frozen human PBMCs, Leukapheresis products, Single-cell suspensions from various tissues samples. To achieve these results, follow this simple 4-step protocol that takes just 25-35 min from start to finish. Furthermore, this streamlined protocol ensures consistent results across experiments.

How the Human CD56 Sorting Magnetic Beads Works

Simple 4-step protocol for rapid cell isolation

Step 1: Sample Preparation

Isolate PBMCs, centrifuge at 300×g for 10 min, and resuspend in Isolation Buffer at a density of 10⁸ MNCs per mL.

Step 2: Magnetic Labeling

Add 10 μL Nanobeads per 10⁷ total MNCs, mix well, incubate at 2–8°C for 15 min, then wash once with buffer and resuspend in 500 μL.

Step 3: Magnetic Separation

Load the cell suspension onto a pre-rinsed column in the magnet, collect the flow-through (CD56− cells), and wash the column three times.

Step 4: Elution

Remove the column from the magnet, add buffer (1 mL for gM / 5 mL for gL), push the plunger to flush out, and collect the CD56+ target cells.

Applications for the Human CD56 Sorting Magnetic Beads

This Human CD56 Sorting Magnetic Beads supports a wide range of downstream applications for CD56+ NK Cell isolation and column-based positive selection workflows from human PBMCs.

Positive enrichment of CD56+ NK cells from human PBMCs for downstream functional studies Isolation of CD56+ NKT cells from PBMCs or leukapheresis products NK cell enrichment for cell culture, expansion, and activation assays Depletion of CD56+ cells from mixed cell populations (negative selection workflow) Downstream applications: flow cytometry analysis, cytotoxicity assays, cytokine profiling, genomic/transcriptomic studies, NK cell therapy research

In addition, the Human CD56 Sorting Magnetic Beads is compatible with a wide range of downstream applications, making it a versatile tool for your immunology research workflow.

Applicable Samples

The Human CD56 Sorting Magnetic Beads is validated for high-purity column-based positive selection from the following Fresh or frozen human PBMCs, Leukapheresis products, Single-cell suspensions from various tissues sample types.

Fresh or frozen human PBMCs
Leukapheresis products
Single-cell suspensions from various tissues

Storage & Shelf Life

Storage

2-8°C

Shelf Life

12 months

Human CD56 Sorting Magnetic Beads — Frequently Asked Questions

Everything you need to know about the MBCS-0009 Human CD56 Sorting Magnetic Beads

Click each question to expand the answer

The MBCS-0009 Human CD56 Sorting Magnetic Beads from Cell Sorts uses column-based positive selection. 10 μL Nanobeads per 10⁷ total MNCs bind CD56+ target cells at 2–8°C for 15 min. A pre-rinsed column in the magnet retains bead-bound CD56+ cells; the flow-through (CD56− cells) is collected. After washing three times, buffer (1 mL for gM / 5 mL for gL) is pushed through with the plunger to elute purified CD56+ target cells.

The MBCS-0009 kit delivers high purity and viability of enriched CD56+ NK cell populations, as validated by flow cytometry using CD56-PE (Clone B159), CD3-APC, CD16-FITC staining. The monoclonal anti-human CD56 antibody (mouse IgG1) ensures high specificity and binding affinity. Purity typically reaches 90–99%.

The MBCS-0009 kit is optimized for human PBMCs. Isolate PBMCs, centrifuge at 300×g for 10 min, and resuspend in Isolation Buffer at a density of 10⁸ MNCs per mL. The kit is compatible with PBMCs from healthy donors and patients with various conditions.

Step 1: Sample Preparation. Isolate PBMCs, centrifuge at 300×g for 10 min, and resuspend in Isolation Buffer at 10⁸ MNCs per mL.
Step 2: Magnetic Labeling. Add 10 μL Nanobeads per 10⁷ total MNCs, mix well, incubate at 2–8°C for 15 min, then wash once with buffer and resuspend in 500 μL.
Step 3: Magnetic Separation. Load onto a pre-rinsed column in the magnet, collect flow-through (CD56− cells), and wash the column three times.
Step 4: Elution. Remove column from magnet, add buffer (1 mL for gM / 5 mL for gL), push plunger to flush out, and collect CD56+ target cells.
Total time: ~30–40 min.

Isolated CD56+ NK cells are immediately ready for: cell culture and NK cell expansion/activation, cancer immunotherapy (CAR-NK development, ADCC assays), innate immunity studies, flow cytometry, functional assays (cytotoxicity, cytokine production), genomic analysis (RNA-seq, scRNA-seq), infectious disease research, and drug discovery. No bead removal is required post-separation — cells are immediately ready for downstream use.

The monoclonal anti-human CD56 antibody (mouse IgG1) ensures high specificity and binding affinity for CD56+ NK cells. CD56 (NCAM) is a well-established surface marker for NK cells, and this antibody clone provides consistent, reliable capture of target cells with minimal off-target binding, resulting in high-purity enriched NK cell populations.

The biodegradable nanoscale matrix (50–100 nm) minimizes steric hindrance and mechanical stress on cells compared to larger microbeads. This ensures efficient antibody-target binding while preserving native surface markers, viability, and functional integrity — critical for downstream cytotoxicity assays and functional studies.

We recommend flow cytometry using CD56-PE (Clone B159), CD3-APC, CD16-FITC antibodies. Typical results show 90–99% CD56+ cells in the positively selected fraction, with minimal CD3+ T cell contamination. Viability can be assessed using Trypan blue exclusion or 7-AAD staining. Since nanobeads do not need to be removed post-separation, no additional washing is required before staining.

Absolutely. Since nanobeads do not need to be removed post-separation, isolated CD56+ NK cells are immediately ready for single-cell RNA sequencing (scRNA-seq) without additional washing. The gentle 50–100 nm nanobeads and streamlined MACS workflow preserve transcriptomic integrity, making the MBCS-0009 kit compatible with 10x Genomics and other major scRNA-seq platforms.

The MBCS-0009 Human CD56 Sorting Magnetic Beads should be stored at 2–8°C and used within 12 months of opening. Do not freeze. The HSA plus Poloxamer 188 stabilized formulation ensures optimal performance and consistent results across experiments.