MBCS-0007

Human CD34 Sorting Magnetic Beads

CD Beads Human peripheral blood, Human cord blood, Human bone marrow, Leukapheresis products

The Cell Sorts MBCS-0007 Human CD34 Sorting Magnetic Beads uses column-based positive selection to enrich and purify CD34+ hematopoietic stem and progenitor cells from human PBMCs. The humanized CD34 monoclonal antibody with significantly reduced Fc receptor affinity ensures high-specificity capture — no additional FcR blocking agent required. Prepare PBMCs, wash with sorting buffer, add CD34 magnetic beads, and incubate at 2–8°C for 15 minutes. Load onto a magnetic column, wash three times with 3 mL sorting buffer, then elute with 5 mL sorting buffer via plunger.

The biodegradable nanoscale matrix (50–100 nm) minimizes steric hindrance and mechanical stress, preserving stem cell viability and functional integrity. Isolated CD34+ cells are ready for cell culture, stem cell research, regenerative medicine, CFU assays, flow cytometry, scRNA-seq, and transplantation research.

Product Specifications

Particle Size

50–100 nm

Sorting Method

Column-Based

Sorting Principle

Positive

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Core Advantages of the Human CD34 Sorting Magnetic Beads

The Human CD34 Sorting Magnetic Beads delivers industry-leading performance for rapid CD34+ Hematopoietic Stem Cell enrichment from Human peripheral blood, Human cord blood, Human bone marrow, Leukapheresis products samples via column-based positive selection.

Humanized CD34 monoclonal antibody with significantly reduced Fc receptor affinity — no additional Fc receptor blocking agent required

Biodegradable nanoscale matrix (50–100 nm) — minimizes steric hindrance and mechanical stress on cells

High-specificity capture — ensures efficient enrichment of CD34+ cells

High-purity isolation of CD34+ hematopoietic stem and progenitor cells

Gentle sorting — preserves cell viability and functional integrity

Streamlined MACS workflow — with established protocols for consistent results

The Human CD34 Sorting Magnetic Beads uses column-based positive selection to enrich purified CD34+ Hematopoietic Stem Cells from Human peripheral blood, Human cord blood, Human bone marrow, Leukapheresis products samples. To achieve these results, follow this simple 4-step protocol that takes just 25-35 min from start to finish. Furthermore, this streamlined protocol ensures consistent results across experiments.

How the Human CD34 Sorting Magnetic Beads Works

Simple 4-step protocol for rapid cell isolation

Step 1: Sample Preparation

Prepare human PBMCs, wash with sorting buffer, centrifuge, and resuspend the cells.

Step 2: Magnetic Labeling

Add CD34 magnetic beads to the cell suspension and incubate at 2–8°C for 15 minutes.

Step 3: Magnetic Separation

Load the mixture onto a magnetic column, wash three times with 3 mL sorting buffer, and collect the flow-through containing unlabeled cells.

Step 4: Elution

Remove the column from the magnet, add 5 mL sorting buffer, immediately push the plunger through, and collect the eluted CD34+ target cells for counting and flow cytometry analysis.

Applications for the Human CD34 Sorting Magnetic Beads

This Human CD34 Sorting Magnetic Beads supports a wide range of downstream applications for CD34+ Hematopoietic Stem Cell isolation and column-based positive selection workflows from human PBMCs.

Positive enrichment and purification of CD34+ hematopoietic stem and progenitor cells (HSPCs) from human peripheral blood Isolation of CD34+ cells from human cord blood, bone marrow, and leukapheresis products CD34+ cell sorting from differentiated ES and iPS cells Downstream applications: cell culture, flow cytometry analysis, colony-forming assays, transplantation research, regenerative medicine studies, genomic/transcriptomic analysis

In addition, the Human CD34 Sorting Magnetic Beads is compatible with a wide range of downstream applications, making it a versatile tool for your immunology research workflow.

Applicable Samples

The Human CD34 Sorting Magnetic Beads is validated for high-purity column-based positive selection from the following Human peripheral blood, Human cord blood, Human bone marrow, Leukapheresis products sample types.

Human peripheral blood
Human cord blood
Human bone marrow
Leukapheresis products

Storage & Shelf Life

Storage

2-8°C

Shelf Life

12 months

Human CD34 Sorting Magnetic Beads — Frequently Asked Questions

Everything you need to know about the MBCS-0007 Human CD34 Sorting Magnetic Beads

Click each question to expand the answer

The MBCS-0007 Human CD34 Sorting Magnetic Beads from Cell Sorts uses column-based positive selection. CD34 magnetic beads are added to the cell suspension and incubated at 2–8°C for 15 minutes. The mixture is then loaded onto a magnetic column, washed three times with 3 mL sorting buffer, and the flow-through containing unlabeled cells is collected. The column is removed from the magnet, 5 mL sorting buffer is added, and the plunger is pushed through to elute purified CD34+ target cells.

The MBCS-0007 kit delivers high-purity isolation of CD34+ hematopoietic stem and progenitor cells, as validated by flow cytometry using CD34-PE (Clone 581), CD38-FITC, CD45-PerCP-Cy5.5 staining. The humanized CD34 monoclonal antibody with significantly reduced Fc receptor affinity ensures high-specificity capture with minimal non-specific carryover. Purity typically reaches 90–99%.

The MBCS-0007 kit is optimized for human PBMCs. Prepare human PBMCs, wash with sorting buffer, centrifuge, and resuspend the cells. The kit is compatible with PBMCs from healthy donors and patients undergoing stem cell mobilization.

Step 1: Sample Preparation. Prepare human PBMCs, wash with sorting buffer, centrifuge, and resuspend the cells.
Step 2: Magnetic Labeling. Add CD34 magnetic beads to the cell suspension and incubate at 2–8°C for 15 minutes.
Step 3: Magnetic Separation. Load the mixture onto a magnetic column, wash three times with 3 mL sorting buffer, and collect the flow-through containing unlabeled cells.
Step 4: Elution. Remove the column from the magnet, add 5 mL sorting buffer, immediately push the plunger through, and collect the eluted CD34+ target cells for counting and flow cytometry analysis.
Total time: ~30–40 min.

Isolated CD34+ cells are immediately ready for: cell culture and HSC expansion/differentiation, stem cell research, regenerative medicine, flow cytometry, functional assays (CFU assays), genomic analysis (RNA-seq, scRNA-seq), drug discovery, and transplantation research. The gentle sorting protocol preserves cell viability and functional integrity essential for stem cell applications.

The humanized CD34 monoclonal antibody with significantly reduced Fc receptor affinity ensures high-specificity capture of CD34+ cells. The reduced FcR affinity means no additional Fc receptor blocking agent is required, simplifying the workflow and reducing reagent costs while maintaining high purity and yield.

The biodegradable nanoscale matrix (50–100 nm) minimizes steric hindrance and mechanical stress on cells compared to larger microbeads. This is especially important for delicate hematopoietic stem and progenitor cells, ensuring efficient antibody-target binding while preserving native surface markers, viability, and stem cell functional potential.

We recommend flow cytometry using CD34-PE (Clone 581), CD38-FITC, CD45-PerCP-Cy5.5 antibodies. The CD34+ fraction should show high purity with characteristic CD34+ CD38− CD45dim expression for hematopoietic stem cells. Viability can be assessed using Trypan blue exclusion or 7-AAD staining. Functional verification can be performed using colony-forming unit (CFU) assays.

Absolutely. Isolated CD34+ cells are immediately ready for single-cell RNA sequencing (scRNA-seq) without additional washing. The gentle 50–100 nm nanobeads and streamlined MACS workflow preserve transcriptomic integrity, making the MBCS-0007 kit compatible with 10x Genomics and other major scRNA-seq platforms.

The MBCS-0007 Human CD34 Sorting Magnetic Beads should be stored at 2–8°C and used within 12 months of opening. Do not freeze. The streamlined MACS workflow with established protocols ensures consistent results across experiments.