MBCS-0006

Human CD3 Sorting Magnetic Beads

CD Beads Fresh or frozen human PBMCs, Leukapheresis products, Single-cell suspensions from tissues

The Cell Sorts MBCS-0006 Human CD3 Sorting Magnetic Beads uses column-based positive selection to enrich and purify CD3+ T cells from human PBMCs. It also supports depletion of CD3+ T cells. The monoclonal anti-human CD3 antibody (mouse IgG2a) ensures high specificity and binding affinity. Isolate PBMCs, centrifuge at 300×g for 10 min, and resuspend in Isolation Buffer at 10⁸ MNCs per mL. Add 10 μL Nanobeads per 10⁷ total MNCs, incubate at 2–8°C for 15 min, then wash and resuspend in 500 μL. Load onto a pre-rinsed column, wash three times, then elute with buffer (1 mL for gM / 5 mL for gL) via plunger.

Nanobeads do not need to be removed post-separation — cells are immediately ready for downstream use. Stabilized formulation with HSA and Poloxamer 188 ensures optimal performance. Isolated CD3+ T cells are ready for cell culture, immunology research, cancer immunotherapy, flow cytometry, scRNA-seq, and drug discovery.

Product Specifications

Particle Size

100 nm

Sorting Method

Column-Based

Sorting Principle

Positive&Negative

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Core Advantages of the Human CD3 Sorting Magnetic Beads

The Human CD3 Sorting Magnetic Beads delivers industry-leading performance for rapid CD3+ T Cell enrichment from Fresh or frozen human PBMCs, Leukapheresis products, Single-cell suspensions from tissues samples via column-based positive selection.

Monoclonal anti-human CD3 antibody (mouse IgG2a) — ensures high specificity and binding affinity for CD3+ T cells

No bead removal required post-separation — cells are immediately ready for downstream use

Supports both enrichment and depletion strategies — optimized bead dosage protocols for each application

Compatible with gM/gL columns and magnets — as well as other compatible column/magnet systems

High purity and viability — of enriched or depleted CD3+ T cell populations

Scalable protocol — 10 μL nanobeads per 10⁷ total MNCs for enrichment; 2–3× amount for depletion

Flexible incubation conditions — 15 min at 2–8°C (standard) or room temperature/30 min for improved depletion efficiency

Stabilized formulation — with HSA and Poloxamer 188 ensures optimal performance

The Human CD3 Sorting Magnetic Beads uses column-based positive selection to enrich purified CD3+ T Cells from Fresh or frozen human PBMCs, Leukapheresis products, Single-cell suspensions from tissues samples. To achieve these results, follow this simple 4-step protocol that takes just 25-35 min from start to finish. Furthermore, this streamlined protocol ensures consistent results across experiments.

How the Human CD3 Sorting Magnetic Beads Works

Simple 4-step protocol for rapid cell isolation

Step 1: Sample Preparation

Isolate PBMCs, centrifuge at 300×g for 10 min, and resuspend in Isolation Buffer at a density of 10⁸ MNCs per mL.

Step 2: Magnetic Labeling

Add 10 μL Nanobeads per 10⁷ total MNCs, mix well, incubate at 2–8°C for 15 min, then wash once with buffer and resuspend in 500 μL.

Step 3: Magnetic Separation

Load the cell suspension onto a pre-rinsed column in the magnet, collect the flow-through (CD3⁻ cells), and wash the column three times.

Step 4: Elution

Remove the column from the magnet, add buffer (1 mL for gM / 5 mL for gL), push the plunger to flush out, and collect the CD3⁺ target cells.

Applications for the Human CD3 Sorting Magnetic Beads

This Human CD3 Sorting Magnetic Beads supports a wide range of downstream applications for CD3+ T Cell isolation and column-based positive selection workflows from human PBMCs.

Positive enrichment of CD3+ T cells from human PBMCs for downstream functional studies, expansion, and activation assays Negative depletion of CD3+ T cells from mixed cell populations Cell isolation from leukapheresis products Single-cell suspension processing from tissues Downstream applications: cell culture, flow cytometry analysis, functional assays (proliferation, cytotoxicity, cytokine profiling), genomic/transcriptomic studies, adoptive T cell therapy research

In addition, the Human CD3 Sorting Magnetic Beads is compatible with a wide range of downstream applications, making it a versatile tool for your immunology research workflow.

Applicable Samples

The Human CD3 Sorting Magnetic Beads is validated for high-purity column-based positive selection from the following Fresh or frozen human PBMCs, Leukapheresis products, Single-cell suspensions from tissues sample types.

Fresh or frozen human PBMCs
Leukapheresis products
Single-cell suspensions from tissues

Storage & Shelf Life

Storage

2-8°C

Shelf Life

12 months

Human CD3 Sorting Magnetic Beads — Frequently Asked Questions

Everything you need to know about the MBCS-0006 Human CD3 Sorting Magnetic Beads

Click each question to expand the answer

The MBCS-0006 Human CD3 Sorting Magnetic Beads from Cell Sorts uses column-based positive selection by default, but also supports depletion of CD3+ T cells. 10 μL Nanobeads per 10⁷ total MNCs bind CD3+ target cells at 2–8°C for 15 min. A pre-rinsed column in the magnet retains bead-bound CD3+ cells; the flow-through (CD3− cells) is collected. After washing three times, buffer (1 mL for gM / 5 mL for gL) is pushed through with the plunger to elute purified CD3+ target cells.

The MBCS-0006 kit delivers high purity and viability of enriched or depleted CD3+ T cell populations, as validated by flow cytometry using CD3-APC, CD4-FITC, CD8a-PerCP-Cy5.5, CD19-PE staining. The monoclonal anti-human CD3 antibody (mouse IgG2a) ensures high specificity and binding affinity. Purity typically reaches 90–99% for positive selection.

The MBCS-0006 kit is optimized for human PBMCs. Isolate PBMCs, centrifuge at 300×g for 10 min, and resuspend in Isolation Buffer at a density of 10⁸ MNCs per mL. The kit is compatible with PBMCs from healthy donors and patients with various conditions.

Step 1: Sample Preparation. Isolate PBMCs, centrifuge at 300×g for 10 min, and resuspend in Isolation Buffer at 10⁸ MNCs per mL.
Step 2: Magnetic Labeling. Add 10 μL Nanobeads per 10⁷ total MNCs, mix well, incubate at 2–8°C for 15 min, then wash once and resuspend in 500 μL.
Step 3: Magnetic Separation. Load onto a pre-rinsed column in the magnet, collect flow-through (CD3− cells), and wash the column three times.
Step 4: Elution. Remove column from magnet, add buffer (1 mL for gM / 5 mL for gL), push plunger to flush out, and collect CD3+ target cells.
Total time: ~30–40 min.

Isolated CD3+ T cells are immediately ready for: cell culture and T cell expansion/activation, immunology research, cancer immunotherapy (CAR-T development), inflammation studies, flow cytometry, functional assays, genomic analysis (RNA-seq, scRNA-seq), and drug discovery. Nanobeads do not need to be removed post-separation — cells are immediately ready for downstream use.

The monoclonal anti-human CD3 antibody (mouse IgG2a) ensures high specificity and binding affinity for CD3+ T cells. This well-characterized antibody clone provides consistent, reliable capture of target cells with minimal off-target binding, resulting in high-purity enriched or depleted cell populations.

Yes. The MBCS-0006 kit supports both enrichment and depletion strategies. For enrichment, use 10 μL nanobeads per 10⁷ total MNCs. For depletion, use 2–3× the standard amount. For improved depletion efficiency, incubate at room temperature for 30 min instead of the standard 15 min at 2–8°C.

We recommend flow cytometry using CD3-APC, CD4-FITC, CD8a-PerCP-Cy5.5, CD19-PE antibodies. Typical results show 90–99% CD3+ cells in the positively selected fraction. Viability can be assessed using Trypan blue exclusion or 7-AAD staining. Since nanobeads do not need to be removed post-separation, no additional washing is required before staining.

Absolutely. Since nanobeads do not need to be removed post-separation, isolated CD3+ T cells are immediately ready for single-cell RNA sequencing (scRNA-seq) without additional washing. The gentle nanobeads and stabilized formulation with HSA and Poloxamer 188 preserve transcriptomic integrity, making the MBCS-0006 kit compatible with 10x Genomics and other major scRNA-seq platforms.

The MBCS-0006 features a stabilized formulation with HSA (Human Serum Albumin) and Poloxamer 188 that ensures optimal performance. HSA provides protein stabilization and reduces non-specific binding, while Poloxamer 188 acts as a surfactant to prevent cell aggregation and maintain bead dispersion. This formulation enhances consistency and reliability across experiments.