MBCS-0003

Human CD14 Sorting Magnetic Beads

CD Beads Human peripheral blood, Umbilical cord blood/pleural fluid/peritoneal fluid/synovial fluid,

The Cell Sorts MBCS-0003 Human CD14 Sorting Magnetic Beads uses column-based positive selection to enrich and purify CD14+ monocytes and macrophages from human PBMCs. It also supports negative selection (depletion) of CD14+ cells. The biodegradable nanoscale matrix (50–100 nm) minimizes steric hindrance and mechanical stress. Resuspend 1×10⁷ cells in 100 μL buffer, add 20 μL CD14 magnetic beads, and incubate at 2–8°C for 15 min. Load onto a MACS column, wash three times with 3 mL buffer, then elute CD14+ cells by pushing buffer through with the plunger.

No column clogging due to nanoscale bead size. Gentle sorting preserves cell viability and functional integrity — immediately ready for cell culture, macrophage differentiation, immunology research, inflammation studies, flow cytometry, scRNA-seq, and drug discovery.

Product Specifications

Particle Size

50–100 nm

Sorting Method

Column-Free

Sorting Principle

Positive&Negative

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Core Advantages of the Human CD14 Sorting Magnetic Beads

The Human CD14 Sorting Magnetic Beads delivers industry-leading performance for rapid CD14+ Cell enrichment from Human peripheral blood, Umbilical cord blood/pleural fluid/peritoneal fluid/synovial fluid, samples via column-free positive selection.

Biodegradable nanoscale matrix (50–100 nm) — minimizes steric hindrance and mechanical stress on cells

Supports both positive selection and negative selection (depletion) of CD14+ cells

High-purity enrichment or efficient depletion of monocytes and macrophages

Gentle sorting — preserves cell viability and functional integrity

Streamlined MACS workflow — established protocols for consistent results

No column clogging — nanoscale bead size prevents column blockage

The Human CD14 Sorting Magnetic Beads uses column-free positive selection to enrich purified CD14+ Cells from Human peripheral blood, Umbilical cord blood/pleural fluid/peritoneal fluid/synovial fluid, samples. To achieve these results, follow this simple 4-step protocol that takes just 18-20 min from start to finish. Furthermore, this streamlined protocol ensures consistent results across experiments.

How the Human CD14 Sorting Magnetic Beads Works

Simple 4-step protocol for rapid cell isolation

Step 1: Sample Preparation

Prepare PBMCs, pass through a 30 μm filter, wash with sorting buffer (PBS pH 7.2, 0.5% BSA, 2 mM EDTA), and centrifuge at 1500 rpm for 5 min.

Step 2: Bead Labeling

Resuspend 1×10⁷ cells in 100 μL buffer, add 20 μL CD14 magnetic beads, and incubate at 2–8°C for 15 min.

Step 3: Magnetic Separation

Equilibrate a MACS column with 3 mL buffer, load the labeled cell suspension, then wash the column three times with 3 mL buffer each to remove unbound CD14⁻ cells.

Step 4: Elution & Analysis

Remove the column from the magnet, add 5 mL buffer, push the plunger to elute CD14⁺ target cells into a 15 mL tube, then count and assess viability and purity by flow cytometry.

Applications for the Human CD14 Sorting Magnetic Beads

This Human CD14 Sorting Magnetic Beads supports a wide range of downstream applications for CD14+ Cell isolation and column-free positive selection workflows from human PBMCs.

Positive enrichment of CD14+ monocytes from human PBMCs for downstream differentiation studies (e.g., dendritic cell or macrophage differentiation) Depletion of CD14+ monocytes/macrophages from mixed cell populations Isolation of monocytes from umbilical cord blood, pleural fluid, peritoneal fluid, or synovial fluid Cell isolation from tissue single-cell suspensions (spleen, lymph node) Downstream applications: cell culture, flow cytometry, functional assays, immunology research

In addition, the Human CD14 Sorting Magnetic Beads is compatible with a wide range of downstream applications, making it a versatile tool for your immunology research workflow.

Applicable Samples

The Human CD14 Sorting Magnetic Beads is validated for high-purity column-free positive selection from the following Human peripheral blood, Umbilical cord blood/pleural fluid/peritoneal fluid/synovial fluid, sample types.

Human peripheral blood
Umbilical cord blood/pleural fluid/peritoneal fluid/synovial fluid

Storage & Shelf Life

Storage

2-8°C

Shelf Life

12 months

Human CD14 Sorting Magnetic Beads — Frequently Asked Questions

Everything you need to know about the MBCS-0003 Human CD14 Sorting Magnetic Beads

Click each question to expand the answer

The MBCS-0003 Human CD14 Sorting Magnetic Beads from Cell Sorts uses column-based positive selection by default, but also supports negative selection (depletion). CD14 magnetic beads (20 μL per 1×10⁷ cells) bind CD14+ target cells at 2–8°C for 15 min. A MACS column retains bead-bound CD14+ cells; after washing three times with 3 mL buffer each, unbound CD14− cells are removed. The column is then removed from the magnet and 5 mL buffer is pushed through with the plunger to elute purified CD14+ target cells.

The MBCS-0003 kit delivers high-purity enrichment or efficient depletion of monocytes and macrophages, as validated by flow cytometry using CD14-PE (Clone M5E2), CD11b-APC, CD16-FITC staining. The biodegradable nanoscale matrix (50–100 nm) ensures efficient binding with minimal non-specific carryover. Purity typically reaches 90–99% for positive selection.

The MBCS-0003 kit is optimized for human PBMCs. Prepare PBMCs, pass through a 30 μm filter, wash with sorting buffer (PBS pH 7.2, 0.5% BSA, 2 mM EDTA), and centrifuge at 1500 rpm for 5 min. The kit is compatible with PBMCs from healthy donors and patients with various conditions.

Step 1: Sample Preparation. Prepare PBMCs, pass through a 30 μm filter, wash with sorting buffer (PBS pH 7.2, 0.5% BSA, 2 mM EDTA), and centrifuge at 1500 rpm for 5 min.
Step 2: Bead Labeling. Resuspend 1×10⁷ cells in 100 μL buffer, add 20 μL CD14 magnetic beads, and incubate at 2–8°C for 15 min.
Step 3: Magnetic Separation. Equilibrate a MACS column with 3 mL buffer, load the labeled cell suspension, then wash the column three times with 3 mL buffer each to remove unbound CD14− cells.
Step 4: Elution & Analysis. Remove the column from the magnet, add 5 mL buffer, push the plunger to elute CD14+ target cells into a 15 mL tube, then count and assess viability and purity by flow cytometry.
Total time: ~30–40 min.

Isolated CD14+ cells are immediately ready for: cell culture and differentiation into macrophages or dendritic cells, immunology research, inflammation studies, infectious disease research, flow cytometry, functional assays, genomic analysis (RNA-seq, scRNA-seq), and drug discovery. The gentle MACS workflow preserves cell viability and functional integrity.

The biodegradable nanoscale matrix (50–100 nm) minimizes steric hindrance and mechanical stress on cells compared to larger microbeads. This ensures efficient antibody-target binding while preserving native cell surface markers, viability, and functional integrity. Additionally, the nanoscale bead size prevents column clogging, ensuring smooth and consistent separation performance.

Yes. The MBCS-0003 kit supports both positive selection and negative selection (depletion) of CD14+ cells. For positive selection, CD14+ cells are retained on the column and eluted. For negative selection (depletion), the protocol can be adapted to collect the CD14− flow-through fraction while CD14+ cells remain on the column. This flexibility makes the kit suitable for a wide range of experimental designs.

We recommend flow cytometry using CD14-PE (Clone M5E2), CD11b-APC, CD16-FITC antibodies. Typical results show 90–99% CD14+ cells (CD14+ CD11b+) in the positively selected fraction. Viability can be assessed using Trypan blue exclusion or 7-AAD staining. For functional verification, monocytes can be differentiated into macrophages and confirmed by CD68 or CD206 expression.

Absolutely. The optional NanoBeads Release Buffer dissociates beads from isolated CD14+ cells, leaving them completely bead-free and ready for single-cell RNA sequencing (scRNA-seq) without additional washing. The gentle 50–100 nm nanobeads preserve transcriptomic integrity, making the MBCS-0003 kit compatible with 10x Genomics and other major scRNA-seq platforms.

The MBCS-0003 Human CD14 Sorting Magnetic Beads should be stored at 2–8°C and used within 12 months of opening. Do not freeze. Vortex gently for 5–30 seconds before each use to ensure uniform bead suspension. The streamlined MACS workflow with established protocols ensures consistent results across experiments.