Human Monocyte Negative Selection Kit CS-0013 | Cell Sorts™
CS-0013

Human Monocyte Sorting Kit

Column-Free Negative Human

Cell Sorts™ Human Monocyte Sorting Kit (CS-0013) enriches an untouched monocyte fraction from density-gradient-prepared human peripheral blood mononuclear cells (PBMCs) by column-free negative selection. Human FcR Blocker and a Human Monocyte Isolation Cocktail prepare and label non-target cells; Streptavidin Nanobeads then enable their magnetic retention. The unlabeled monocyte-enriched fraction is recovered from the supernatant by controlled pour-off, without a separation column.

The documented workflow uses pre-chilled FACS buffer, a 15-minute cocktail incubation at 4°C, a 400 × g wash for 5 minutes, a 15-minute nanobead incubation at 4°C, and a 3–5-minute magnetic hold. One representative CD11b/CD14 flow-cytometry example increased the monitored monocyte fraction from 16.9% before separation to 90.7% afterward; this is not a guaranteed specification. Confirm identity, purity, viability, recovery, and functional suitability for every donor and downstream assay. For research use only.

Sorting Purity
Representative result 90.7%
Sorting Time
Approximately 38-40 min timed steps after PBMC preparation

Product Features of the Human Monocyte Sorting Kit

CS-0013 uses a documented column-free negative-selection workflow to enrich an untouched human monocyte fraction from PBMCs using pre-chilled buffer, two 15-minute incubations, a 400 × g wash, and 3-5 minutes of magnetic capture.

Column-free negative selection - Labeled non-monocytes are magnetically retained without a separation column
Untouched target fraction - The unlabeled monocyte-enriched fraction is recovered from the supernatant by controlled pour-off
Defined cold workflow - The documented method uses pre-chilled FACS buffer and two 15-minute incubations at 4°C
Documented wash step - A ten-volume buffer wash and 400 × g centrifugation for 5 minutes precede nanobead labeling
Format-specific magnetic hold - Use 3 minutes with a single-tube separator or 5 minutes with a multi-tube rack
Evidence-qualified performance - One representative CD11b/CD14 example increased from 16.9% to 90.7%; results are sample- and workflow-dependent

The Human Monocyte Sorting Kit uses column-free negative selection to isolate untouched Untouched Human Monocytes (CD11b+/CD14+ Enriched Fraction) from human PBMCs. Review the product-specific four-stage workflow below. The stated sorting time of Approximately 38-40 min timed steps after PBMC preparation covers the specified timed stages only; sample preparation, handling, optional collection, and quality control require additional time. Follow the current product instructions and validate each preparation before downstream use.

How the Human Monocyte Sorting Kit Works

Four stages summarizing the documented PBMC monocyte-enrichment workflow

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CS-0013 workflow step 1: prepare and count density-gradient human PBMCs in pre-chilled FACS buffer

Step 1: Prepare and Count PBMCs

Prepare density-gradient human PBMCs, count cells, and resuspend in pre-chilled FACS buffer at no more than 1×10⁸ cells/mL. Red-blood-cell lysis is not recommended for this PBMC preparation.

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CS-0013 workflow step 2: block Fc receptors and label non-monocyte cells for 15 minutes at 4°C

Step 2: Block and Label Non-Monocytes

Add Human FcR Blocker and Human Monocyte Isolation Cocktail according to the current scale table, mix gently, and incubate for 15 minutes at 4°C.

3
CS-0013 workflow step 3: wash at 400 × g and bind Streptavidin Nanobeads for 15 minutes at 4°C

Step 3: Wash and Bind Nanobeads

Add ten volumes of pre-chilled FACS buffer, centrifuge at 400 × g for 5 minutes, restore the original volume, add the scale-table dose of Streptavidin Nanobeads, and incubate for 15 minutes at 4°C.

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CS-0013 workflow step 4: magnetically retain labeled cells and pour off the untouched monocyte-enriched fraction

Step 4: Capture and Recover Monocytes

Add at least five volumes of pre-chilled buffer, hold for 3 minutes in a single-tube separator or 5 minutes in a multi-tube rack, and pour the untouched monocyte-enriched fraction into a new tube.

Applications for the Human Monocyte Sorting Kit

Potential downstream uses for the enriched untouched monocyte fraction are listed below. Confirm CD11b/CD14 identity, purity, viability, recovery, and functional suitability under laboratory-specific conditions.

Flow Cytometric Quality Control - Assess CD11b/CD14 enrichment, viability, recovery, and residual populations with a laboratory-validated panel Monocyte Subset Phenotyping - Characterize classical, intermediate, or non-classical monocyte subsets with an assay-appropriate marker panel Phagocytosis and Chemotaxis Research - Use qualified monocyte preparations in controlled innate-immune functional assays Cytokine and Ex Vivo Stimulation Studies - Evaluate responses to defined stimuli after confirming sample identity and quality Macrophage Differentiation Research - Establish laboratory-validated differentiation workflows after post-sort count, viability, and purity checks Dendritic-Cell Differentiation Research - Prepare monocyte inputs for controlled differentiation studies using validated culture conditions Molecular and Single-Cell Profiling - Prepare DNA, RNA, protein, or single-cell inputs when platform-specific acceptance criteria are met Sample-Preparation and Separation Development - Optimize PBMC preparation, buffer temperature, reagent scaling, magnetic capture, and fraction recovery

The listed applications are potential research uses, not guaranteed performance claims. Confirm post-sort identity, purity, viability, recovery, and functional suitability under laboratory-specific conditions.

Kit Components

CS-0013 includes the documented Human FcR Blocker, Human Monocyte Isolation Cocktail, and Streptavidin Nanobeads. Pre-chilled FACS buffer, compatible sterile tubes and magnetic equipment, and a centrifuge are required separately.

  • Human FcR Blocker
  • Human Monocyte Isolation Cocktail
  • Streptavidin Nanobeads

Storage & Shelf Life

Storage
4°C; avoid freezing
Shelf Life
Refer to the product label

Human Monocyte Sorting Kit — Frequently Asked Questions

Everything you need to know about the CS-0013 Human Monocyte Sorting Kit

Click each question to expand the answer

CS-0013 enriches an untouched human monocyte fraction from density-gradient-prepared human PBMCs. The supplied representative quality-control example uses CD11b and CD14 to assess the monitored monocyte population.
The kit uses column-free negative selection. Human FcR Blocker and the Human Monocyte Isolation Cocktail support labeling of non-target cells, Streptavidin Nanobeads bind the labeled fraction, and a compatible tube magnet retains those cells while the unlabeled monocyte-enriched suspension is poured into a new tube.
Use human PBMCs prepared by density-gradient separation, count the cells, and resuspend them in pre-chilled FACS buffer at no more than 1×108 cells/mL. The supplied procedure advises against red-blood-cell lysis for PBMC preparation.
The scale table lists 1×107 cells in 100 µL, with 10 µL Human Monocyte Isolation Cocktail and 10 µL Streptavidin Nanobeads. Dose the FcR blocker and all reagents according to the current pack insert and the applicable scale table.
Use pre-chilled FACS buffer consisting of 1× PBS, 2% FBS, and 0.02% EDTA. The documented cocktail and nanobead incubations are each 15 minutes at 4°C. Keep cells and reagents under the current handling conditions throughout the workflow.
Yes. After cocktail labeling, add ten volumes of pre-chilled FACS buffer, centrifuge at 400 × g for 5 minutes, discard the supernatant, and restore the original suspension volume before adding nanobeads. An optional 400 × g, 10-minute centrifugation may be used after collection.
After adding at least five volumes of pre-chilled buffer, place the tube on the magnet immediately. The supplied procedure specifies 3 minutes for a single-tube magnetic separator or 5 minutes for a multi-tube magnetic rack, followed by controlled pour-off into a fresh tube.
The supplied document describes approximate purity of at least 85% and results up to 95%. In one representative CD11b/CD14 flow-cytometry example, the monitored population increased from 16.9% before separation to 90.7% afterward. These are evidence examples, not guaranteed specifications.
The documented components are Human FcR Blocker, Human Monocyte Isolation Cocktail, and Streptavidin Nanobeads. The laboratory must supply pre-chilled FACS buffer, compatible sterile tubes and magnetic equipment, a centrifuge, and any flow-cytometry or downstream-assay reagents.
Store the supplied components at 4°C and avoid freezing. CS-0013 is for research use only. Confirm monocyte identity, purity, viability, recovery, and functional suitability for every donor, sample-preparation method, and downstream assay.

Related topics covered:

column-free negative selection Untouched Human Monocytes (CD11b+/CD14+ Enriched Fraction) isolation Untouched Human Monocytes (CD11b+/CD14+ Enriched Fraction) purity verification Untouched Human Monocytes (CD11b+/CD14+ Enriched Fraction) sorting protocol column-free isolation single-cell analysis (scRNA-seq) Untouched Human Monocytes (CD11b+/CD14+ Enriched Fraction) isolation markers PBMC Untouched Human Monocytes (CD11b+/CD14+ Enriched Fraction) sorting

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