CS-0024

Human CD14 NanoBeads Sorting Kit

Column-Based Positive Human

The Human CD14 NanoBeads Sorting Kit uses column-based positive selection to isolate CD14+ monocytes from human PBMCs with 95–99% purity in 20–30 min. Anti-human CD14 NanoBeads label CD14+ cells, which are retained in a magnetic column while lymphocytes and granulocytes flow through. Eluted cells are ready for macrophage differentiation, dendritic cell generation, phagocytosis assays, and cytokine profiling.

Compared to density gradient centrifugation (40–60% purity) or FACS (expensive, low throughput), this kit delivers 95–99% CD14+ purity with >90% recovery and >98% viability. The releasable NanoBeads leave cells bead-free in 10 min — ideal for single-cell RNA sequencing (scRNA-seq), inflammasome studies, and monocyte-derived macrophage cultures.

Sorting Purity

>95%

Sorting Time

30–40 min

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Product Features of the Human CD14 NanoBeads Sorting Kit

The Human CD14 NanoBeads Sorting Kit delivers industry-leading performance for rapid CD14+ Monocytes isolation from PBMCs via column-based positive selection.

High purity >95% — Advanced negative selection for ultra-pure cell populations

Fast protocol — Complete isolation in just 30–40 min

Column-assisted separation — Magnetic column enables efficient depletion with high recovery rate

No bead residue — Isolated cells are bead-free and ready for immediate downstream use

Preserved functionality — Antibodies and beads do not contact target cells, maintaining their original functional state

The Human CD14 NanoBeads Sorting Kit uses column-based positive selection to isolate purified CD14+ Monocytes from human PBMCs. To achieve these results, follow this simple 4-step protocol that takes just 30–40 min from start to finish. No centrifugation and no lengthy incubations. Furthermore, this streamlined protocol ensures consistent results across experiments.

How the Human CD14 NanoBeads Sorting Kit Works

Simple 4-step protocol for rapid cell isolation

Step 1: Sample Preparation

Prepare a PBMC single-cell suspension from human blood. Count cells and resuspend at 1×10⁸ cells/mL in cold separation buffer. Add Human FcR Blocker to prevent non-specific binding.

Step 2: NanoBeads Labeling

Add anti-CD14 NanoBeads directly to the cell suspension and incubate at 4°C for 10–15 min. Nano-sized magnetic beads bind to CD14+ monocytes with high specificity.

Step 3: Magnetic Column Retention

Load the cell-bead mixture onto a pre-washed magnetic separation column. CD14+ monocytes are retained in the column, while unlabeled non-target cells flow through.

Step 4: Target Cell Elution

Remove the column from the magnet and elute retained CD14+ monocytes by plunger press. Optionally release NanoBeads for bead-free monocytes ready for downstream differentiation.

Applications for the Human CD14 NanoBeads Sorting Kit

This Human CD14 NanoBeads Sorting Kit supports a wide range of downstream applications for CD14+ Monocytes isolation and column-based positive selection workflows from human PBMCs.

Monocyte Biology Research — Phagocytosis, chemotaxis, cytokine production, and differentiation studies Macrophage Differentiation — Source of monocytes for in vitro M1/M2 macrophage polarization protocols Dendritic Cell Generation — CD14+ monocytes as precursors for in vitro DC differentiation Inflammation Research — Monocyte-mediated inflammatory response and signaling studies Infectious Disease Research — Pathogen-monocyte interaction and innate immunity studies Flow Cytometry — High-purity CD14+ cell populations ready for multi-parameter staining and analysis Drug Discovery — Monocyte-targeted drug screening and anti-inflammatory compound testing

In addition, the Human CD14 NanoBeads Sorting Kit is compatible with a wide range of downstream applications, making it a versatile tool for your immunology research workflow.

Kit Components

Each Human CD14 NanoBeads Sorting Kit contains everything needed for high-purity column-based positive selection from human PBMCs.

Human CD14 Nano Beads
User manual

Storage & Shelf Life

Storage

Store at 2-8°C protected from light

Shelf Life

12 months

Human CD14 NanoBeads Sorting Kit — Frequently Asked Questions

Everything you need to know about the CS-0024 Human CD14 NanoBeads Sorting Kit

Click each question to expand the answer

The CS-0024 Human CD14 NanoBeads Sorting Kit uses column-based positive selection. CD14+ monocytes are directly labeled with NanoBeads and retained in the magnetic column, while non-target cells pass through. This delivers 95–99% purity of viable, functional CD14+ monocytes from human PBMCs.

Purity of isolated human CD14+ monocytes typically reaches 95–99%, as validated by flow cytometry using anti-human CD14 antibody staining. The NanoBeads positive selection protocol efficiently enriches monocytes while depleting lymphocytes and granulocytes.

The entire column-based positive selection protocol takes approximately 20–30 minutes from start to finish: 10–15 min for NanoBeads labeling, 5 min for column washing, and 5–10 min for target cell elution.

The CS-0024 kit uses nano-sized magnetic beads (NanoBeads) that offer higher binding efficiency and lower non-specific binding compared to conventional micron-sized beads. Their small size ensures minimal interference with cell surface markers, and they are fully releasable—leaving monocytes bead-free for downstream differentiation applications.

Step 1: Prepare a PBMC single-cell suspension from human blood.
Step 2: Incubate with anti-CD14 NanoBeads positive selection cocktail at 4°C for 10–15 min.
Step 3: Pass through magnetic column to retain labeled CD14+ monocytes.
Step 4: Elute purified CD14+ monocytes from the column.
Step 5 (optional): Release NanoBeads from cell surface.
Total time: 20–30 minutes.

Isolated human CD14+ monocytes are immediately ready for: macrophage differentiation (M1/M2 polarization), dendritic cell generation (GM-CSF + IL-4), osteoclast formation (M-CSF + RANKL), phagocytosis assays, cytokine production studies (TNF-α, IL-6, IL-10), inflammasome activation studies, bacterial and viral infection models, CRISPR gene editing, single-cell RNA sequencing (scRNA-seq), and drug screening for monocyte-targeted therapies.

Yes, isolated CD14+ monocytes are ideal for differentiation studies. Human monocytes can be differentiated into: (1) Macrophages using M-CSF (50 ng/mL), (2) Dendritic cells using GM-CSF (100 ng/mL) + IL-4 (20 ng/mL), and (3) Osteoclasts using M-CSF + RANKL (50 ng/mL each). The bead-free, high-purity monocytes ensure consistent and reproducible differentiation results.

The positive selection cocktail targets CD14, a glycoprotein highly expressed on monocytes and macrophages. This marker effectively distinguishes monocytes from lymphocytes (T cells, B cells, NK cells) and granulocytes in human PBMCs.

We recommend flow cytometry using anti-human CD14-FITC, CD11b-APC, CD16-PE, and HLA-DR-PerCP-Cy5.5 antibodies. Typical results show >95% CD14+CD11b+ cells. Additional markers (CD16+ for inflammatory monocytes, CD16- for classical monocytes) can be used for subset characterization. Viability can be assessed using Trypan blue or 7-AAD staining.

Absolutely. Since isolated monocytes are bead-free after NanoBeads release, they are immediately compatible with single-cell RNA sequencing (scRNA-seq) and other single-cell technologies. This enables high-resolution profiling of monocyte heterogeneity (classical, intermediate, non-classical subsets) and their differentiation trajectories.