Human CD8+ T Cell Sorting Kit CS-0008 for column-based T cell isolation | Cell Sorts™
CS-0008

Human CD8+ T Cell Sorting Kit

Column-Based Negative Human

Cell Sorts™ Human CD8+ T Cell Sorting Kit (CS-0008) enriches untouched CD8+ T cells from human PBMCs by column-based negative selection. Non-CD8 cells are tagged with a biotinylated antibody cocktail and magnetic nanobeads, then retained inside a compatible magnetic column. Unlabeled CD8+ T cells pass through the column by gravity and are collected as the target fraction.

The documented purity range is 80–93% under tested conditions. Available kit formats provide reagents for total starting-cell inputs of up to 1×10⁸, 5×10⁸, or 1×10⁹ cells. Because one L-format column has a documented maximum load of 1×10⁸ cells, larger inputs require a planned multi-column workflow. Actual performance depends on PBMC quality, labeling, column loading, flow behavior, and fraction collection. For research use only.

Sorting Purity
80–93% under tested conditions
Sorting Time
Not specified

Product Features of the Human CD8+ T Cell Sorting Kit

The Human CD8+ T Cell Sorting Kit delivers industry-leading performance for rapid CD8+ T Cell isolation from PBMCs via column-based negative selection.

Column-based negative selection — Magnetically labeled non-target cells are retained in a compatible L column
Untouched CD8+ T-cell fraction — Target cells are collected in the flow-through without direct antibody or bead labeling
Documented purity 80–93% under tested conditions — Performance varies with donor biology, PBMC quality, and column handling
Defined loading limit — One L column accommodates up to 1×10⁸ total cells with a minimum sample-loading volume of 500 µL
Specified column preparation — Equilibrate with 3 mL buffer and wash with 3 mL buffer to recover the enriched fraction
Protocol timing reported by step — The technical documentation does not state a validated total processing time

CS-0008 uses column-based negative selection to enrich untouched human CD8+ T cells from PBMCs. The technical documentation defines the individual incubation, centrifugation, column-equilibration, loading, and wash steps but does not state a validated total processing time.

How the Human CD8+ T Cell Sorting Kit Works

Simple 4-step protocol for rapid cell isolation

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Step 1 of CD8+ T cell sorting: Sample Preparation - Prepare PBMC single-cell suspension with FcR Blocker for CS-0008 Human CD8+ T Cell Sorting Kit

Step 1: Prepare the PBMC Suspension

Prepare a viable single-cell PBMC suspension and resuspend 1×10⁷ cells in 100 µL of the recommended separation buffer. Scale reagent volumes proportionally for larger samples.

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Step 2 of CD8+ T cell sorting: Antibody Cocktail Labeling - Biotin-conjugated antibodies target non-CD8+ cells for depletion with CS-0008

Step 2: Label and Wash Non-Target Cells

Add 10 µL of isolation cocktail per 1×10⁷ cells and incubate for 10 minutes at room temperature. Add 10× buffer volume, centrifuge at 400×g for 5 minutes, discard the supernatant, and restore the original volume.

3
Step 3 of CD8+ T cell sorting: Magnetic Nanobead Binding - Streptavidin nanobeads bind labeled non-target cells for magnetic retention with CS-0008

Step 3: Bind Nanobeads and Prepare the Column

Add 10 µL of nanobeads per 1×10⁷ cells and incubate for 5 minutes at room temperature. In parallel, equilibrate a compatible L column with 3 mL of buffer.

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Step 4 of CD8+ T cell sorting: Column Separation - Untouched CD8+ cytotoxic T cells collected by gravity flow for CTL assays with CS-0008

Step 4: Load, Separate, and Collect

Adjust the sample to at least 500 µL and do not exceed 1×10⁸ cells per L column. Load by gravity, collect the CD8+ T-cell flow-through, then wash the column with 3 mL of buffer and combine the fractions.

Applications for the Human CD8+ T Cell Sorting Kit

This Human CD8+ T Cell Sorting Kit supports a wide range of downstream applications for CD8+ T Cell isolation and column-based negative selection workflows from human PBMCs.

Flow Cytometry — Enrich CD8+ T cells before subset and activation-marker analysis Cell Culture — Collect an untouched flow-through population for culture after post-isolation QC Functional CD8+ T-Cell Assays — Support validated activation, proliferation, cytokine, or cytotoxicity experiments Column-Process Development — Examine how input, column loading, flow behavior, washing, and collection affect purity and recovery Fraction-Based Troubleshooting — Compare the target flow-through and retained fraction when optimizing the separation Repeatable Column Workflows — Standardize column preparation and fraction tracking across donor samples

In addition, the Human CD8+ T Cell Sorting Kit is compatible with a wide range of downstream applications, making it a versatile tool for your immunology research workflow.

Kit Components

Each Human CD8+ T Cell Sorting Kit contains everything needed for high-purity column-based negative selection from human PBMCs.

  • Human CD8+ T Cell Isolation Cocktail
  • Magnetic Nanobeads
  • User Manual

Storage & Shelf Life

Storage
Store at 2-8°C protected from light
Shelf Life
12 months

Human CD8+ T Cell Sorting Kit — Frequently Asked Questions

Everything you need to know about the CS-0008 Human CD8+ T Cell Sorting Kit

Click each question to expand the answer

CS-0008 is intended for enrichment of untouched human CD8+ T cells from PBMC preparations. Sample viability, clumping, donor variability, starting composition, and cell count can influence purity and recovery.
A depletion cocktail and magnetic nanobeads label non-target cells. The sample is loaded onto a compatible magnetized L column, which retains labeled cells while untouched CD8+ T cells are collected in the flow-through and wash fractions.
No. CS-0008 uses negative selection, so depletion reagents are directed to non-target cells. The recovered CD8+ T-cell population is collected without direct antibody or bead labeling.
The supplied technical documentation reports 80–93% purity under tested conditions. Results are sample- and process-dependent, so purity, viability, recovery, and yield should be measured for each laboratory workflow.
No validated total processing time is stated in the supplied documentation. It specifies a 10-minute cocktail incubation, a 5-minute nanobead incubation, a 5-minute centrifugation, and the required column-equilibration, loading, and wash steps.
The documented maximum is 1×10⁸ total cells per L column. Overloading can impair retention of labeled cells and reduce enrichment performance. Use additional columns when processing larger samples.
Adjust the labeled sample to a minimum loading volume of 500 µL before applying it to the equilibrated L column. Maintain a single-cell suspension and follow the current instructions for use.
Equilibrate the compatible L column with 3 mL of the recommended buffer. After collecting the initial flow-through, wash with 3 mL of buffer and combine the fractions as directed.
Review PBMC viability and aggregation, cell counting, reagent scaling, incubation and wash execution, centrifugation conditions, column equilibration, loading capacity, gravity flow, and fraction collection. Confirm performance with an appropriate flow-cytometry panel.
Select CS-0008 when a defined column-based negative-selection workflow is required and compatible L-column equipment is available. Select CS-0007 when a column-free tube-magnet workflow is preferred. Evaluate each format against sample scale, equipment, recovery, and internal validation needs.

Related topics covered:

column-based negative selection CD8+ T Cell isolation CD8+ T Cell purity verification CD8+ T Cell sorting protocol column-based vs column-free single-cell analysis (scRNA-seq) CD8+ T Cell isolation markers PBMC CD8+ T Cell sorting

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